Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit

Runshan Will Jiang; Karol Jaroch; Janusz Pawliszyn

doi:10.1016/j.jpha.2022.09.002

Volume 13 Issue 1

Jan. 2023

Turn off MathJax

Article Contents

Article Navigation > Journal of Pharmaceutical Analysis > 2023 > 13(1): 55-62

Runshan Will Jiang, Karol Jaroch, Janusz Pawliszyn. Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit[J]. Journal of Pharmaceutical Analysis, 2023, 13(1): 55-62. doi: 10.1016/j.jpha.2022.09.002

Citation:

Runshan Will Jiang, Karol Jaroch, Janusz Pawliszyn. Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit[J]. Journal of Pharmaceutical Analysis, 2023, 13(1): 55-62. doi: 10.1016/j.jpha.2022.09.002

Citation:

Runshan Will Jiang, Karol Jaroch, Janusz Pawliszyn. Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit[J]. Journal of Pharmaceutical Analysis, 2023, 13(1): 55-62. doi: 10.1016/j.jpha.2022.09.002

PDF( 1082 KB)

Solid-phase microextraction of endogenous metabolites from intact tissue validated using a Biocrates standard reference method kit

doi: 10.1016/j.jpha.2022.09.002

a. Department of Chemistry, University of Waterloo, Waterloo, N2L 3G1, Canada;
b. Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Toruń, Bydgoszcz, 85-089, Poland

Funds:

The authors would like to thank Biocrates Life Sciences (Innsbruck, Austria) for providing the AbsoluteIDQ p180 kit as well as their technical support. This work was supported by the Natural Sciences and Engineering Research Council of Canada, NSERC (Grant No.: IRCPJ 184412-15).

Received Date: Apr. 18, 2022
Accepted Date: Sep. 22, 2022
Rev Recd Date: Sep. 22, 2022
Publish Date: Oct. 08, 2022

Abstract

Abstract

Improved analytical methods for the metabolomic profiling of tissue samples are constantly needed. Currently, conventional sample preparation methods often involve tissue biopsy and/or homogenization, which disrupts the endogenous metabolome. In this study, solid-phase microextraction (SPME) fibers were used to monitor changes in endogenous compounds in homogenized and intact ovine lung tissue. Following SPME, a Biocrates AbsoluteIDQ assay was applied to make a downstream targeted metabolomics analysis and confirm the advantages of in vivo SPME metabolomics. The AbsoluteIDQ kit enabled the targeted analysis of over 100 metabolites via solid-liquid extraction and SPME. Statistical analysis revealed significant differences between conventional liquid extractions from homogenized tissue and SPME results for both homogenized and intact tissue samples. In addition, principal component analysis revealed separated clustering among all the three sample groups, indicating changes in the metabolome due to tissue homogenization and the chosen sample preparation method. Furthermore, clear differences in free metabolites were observed when extractions were performed on the intact and homogenized tissue using identical SPME procedures. Specifically, a direct comparison showed that 47 statistically distinct metabolites were detected between the homogenized and intact lung tissue samples (P < 0.05) using mixed-mode SPME fibers. These changes were probably due to the disruptive homogenization of the tissue. This study's findings highlight both the importance of sample preparation in tissue-based metabolomics studies and SPME's unique ability to perform minimally invasive extractions without tissue biopsy or homogenization while providing broad metabolite coverage.
- Solid-phase microextraction,
- Solvent extraction,
- Metabolomics,
- Sample preparation,
- In vivo sampling

FullText(HTML)

References(30)

References

T. Nagele, Linking metabolomics data to underlying metabolic regulation, Front. Mol. Biosci. 1 (2014) 1-6

A. Roszkowska, M. Yu, V. Bessonneau, et al., Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach, Sci. Rep. 8 (2018), 6980

M. Yu, S. Lendor, A. Roszkowska, et al., Metabolic profile of fish muscle tissue changes with sampling method, storage strategy and time, Anal. Chim. Acta 1136 (2020) 42-50

V. Hernandes, C. Barbars, D. Dudzik, A review of blood sample handling and pre-processing for metabolomics studies, Electrophoresis. 38 (2017) 2232-2241

D. Vuckovic, I. de Lannoy, B. Gien, et al., In vivo solid-phase microextraction: capturing the elusive portion of metabolome, Angewandte. 50 (2011) 5344-5348

G. Ouyang, D. Vuckovic, J. Pawliszyn, Nondestructive sampling of living systems using in vivo solid-phase microextraction, Chem. Rev. 111 (2011) 2784-2814

E. Boyaci, B. Bojko, N. Reyes-Garces, et al., High-throughput analysis using non-depletive SPME: challenges and applications to the determination of free and total concentrations in small sample volumes, Sci. Rep. 8 (2018), 1167

A. Napylov, N. Reyes-Garces, G. Gomez-Rios, et al., In vivo solid-phase microextraction for sampling of oxylipins in brain of awake, moving rats, Angewandte. 59 (2020) 2392-2398

K. Burlikowska, I. Stryjak, J. Bogusiewicz, et al., Comparison of metabolomic profiles of organs in mice of different strains based on SPME-LC-HRMS, Metabolites. 10 (2020), 255

S. Hassani, S. Lendor, A. Neumann, et al., Dose Dependent Dissociation of Pro-cognitive Effects of Donepezil on Attention and Cognitive Flexibility in Rhesus Monkeys, 2021. https://doi.org/10.1016/j.bpsgos.2021.11.012

J. Bogusiewicz, K. Burlikowska, K. Luczykowski, et al., New chemical biopsy tool for spatially resolved profiling of human brain tissue in vivo, Sci. Rep. 11 (2021), 19522

B. Bojko, N. Looby, M. Olkowicz, et al., Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion, J. Pharm. Anal. 11 (2021) 37-47

P. Dos Santos, J. Sakamoto, M. Chen, et al., Modified in vivo lung perfusion for local chemotherapy: a preclinical study with doxorubicin, Ann. Thorac. Surg. 101 (2016) 2132-2140

F. Musteata, I. de Lannoy, B. Gien, et al., Blood sampling without blood draws for in vivo pharmacokinetic studies in rats, J. Pharm. Biomed. Anal. 47 (2008) 907-912

F. Musteata, M. Musteata, J. Pawliszyn, Fast in vivo microextraction: a new tool for clinical analysis, Clin. Chem. 52 (2006) 708-715

A. Roszkowska, M. Tascon, B. Bojko, et al., Equilibrium ex vivo calibration of homogenized tissue for in vivo SPME quantitation of doxorubicin in lung tissue, Talanta. 183 (2018) 304-310

I. Gertsman, B. Barshop, Promises and pitfalls of untargeted metabolomics, J. Inherit. Metab. Dis. 41 (2018) 355-366

J. Thompson, K. Adams, J Adamski, et al., International ring trial of a high resolution targeted metabolomics and lipidomics platform for serum and plasma analysis, Anal. Chem. 91 (2019) 14407-14416

S. Zukunft, C. Prehn, C. Rohring, et al., High-throughput extraction and quantification method for targeted metabolomics in murine tissues, Metabolomics. 14 (2018), 18

M. Huq, M. Tascon, E. Nazdrajic, et al., Measurement of free drug concentration from biological tissue by solid-phase microextraction: in Silico and experimental study, Anal. Chem. 91 (2019) 7719-7728

M. Alam, J. Pawliszyn, Effect of binding components in complex sample matrices on recovery in direct immersion solid-phase microextraction: friends or foe? Anal. Chem. 90 (2018) 2430-2433

N. Reyes-Garces, E. Gionfriddo, G. Gomez-Rios, et al., Advances in solid phase microextraction and perspective on future directions, Anal. Chem. 90 (2017) 302-360

S. Law, M. Chan, G. Marathe, et al., An updated review of lysophosphatidylcholine metabolism in human diseases, Int. J. Mol. Sci. 20 (2019), 1149

C. Indiveri, V. Iacobazzi, A. Tonazzi, et al., The mitochondrial carnitine/acylcarnitine carrier: function, structure and physiopathology, Mol. Aspect. Med. 32 (2011) 223-233

E. North, D. Osborne, P. Bridson, et al., Autotaxin structure-activity relationships revealed through lysophosphatidylcholine analogs, Bioorg. Med. Chem. 17 (2009) 3433-3442

F. Mansour, W. Wei, N. Danielson, Separation of carnitine and acylcarnitines in biological samples: a review, Biomed. Chromatogr. 27 (2013) 1339-1353

P. Rinaldo, T. Cowan, D. Matern, Acylcarnitine profile analysis, Genet. Med. 10 (2008) 151-156

M. Kaplan, R. Simoni, Intracellular transport of phosphatidylcholine to the plasma membrane, J. Cell Biol. 101 (1985) 441-445

A. Birjandi, B. Bojko, Z. Bing, et al., High throughput solid phase microextraction: a new alternative for analysis of cellular lipidome? J. Chromatogr., B 1043 (2017) 12-19

L. Wu, Z. Yuan, B. Yang, et al., In vivo solid-phase microextraction swab-mass spectrometry for multidimensional analysis of human saliva, Anal. Chim. Acta 1164 (2021), 338510

Relative Articles

Supplements(0)

Cited By

Proportional views