Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification

Liu Liu; Juncheng Hong; Wenhai Wang; Shu Xiao; Hongzhen Xie; Qiqin Wang; Ning Gan

doi:10.1016/j.jpha.2022.07.001

Volume 12 Issue 6

Dec. 2022

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Liu Liu, Juncheng Hong, Wenhai Wang, Shu Xiao, Hongzhen Xie, Qiqin Wang, Ning Gan. Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification[J]. Journal of Pharmaceutical Analysis, 2022, 12(6): 913-922. doi: 10.1016/j.jpha.2022.07.001

Citation:

Liu Liu, Juncheng Hong, Wenhai Wang, Shu Xiao, Hongzhen Xie, Qiqin Wang, Ning Gan. Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification[J]. Journal of Pharmaceutical Analysis, 2022, 12(6): 913-922. doi: 10.1016/j.jpha.2022.07.001

Citation:

Liu Liu, Juncheng Hong, Wenhai Wang, Shu Xiao, Hongzhen Xie, Qiqin Wang, Ning Gan. Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification[J]. Journal of Pharmaceutical Analysis, 2022, 12(6): 913-922. doi: 10.1016/j.jpha.2022.07.001

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Fluorescent aptasensor for detection of live foodborne pathogens based on multicolor perovskite-quantum-dot-encoded DNA probes and dual-stirring-bar-assisted signal amplification

doi: 10.1016/j.jpha.2022.07.001

a. School of Material Science and Chemical Engineering, Ningbo University, Ningbo, Zhejiang, 315211, China;
b. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant No.: 21974074), Ningbo Public Welfare Technology Plan Project of China (Grant Nos.: 2021Z056, 2022Z170, 2022S011, and 202002N3112), Zhejiang Provincial Top Discipline of Biological Engineering (Level A) (Grant Nos.: CX2021051 and KF2021004), Zhejiang Province Public Welfare Technology Application Research Analysis Test Plan (Grant No.: LGC20B050006), and K.C. Wong Magna Fund in Ningbo University.

Received Date: Mar. 09, 2022
Accepted Date: Jul. 09, 2022
Rev Recd Date: Jul. 04, 2022
Publish Date: Dec. 26, 2022

Abstract

Abstract

In this study, a fluorescent (FL) aptasensor was developed for on-site detection of live Salmonella typhimurium (S.T.) and Vibrio parahaemolyticus (V.P.). Complementary DNA (cDNA) of aptamer (Apt)-functionalized multicolor polyhedral oligomeric silsesquioxane-perovskite quantum dots (cDNA-POSS-PQDs) were used as encoded probes and combined with dual-stirring-bar-assisted signal amplification for pathogen quantification. In this system, bar 1 was labeled with the S.T. and V.P. Apts, and then bar 2 was functionalized with cDNA-POSS-PQDs. When S.T. and V.P. were introduced, pathogen-Apt complexes would form and be released into the supernatant from bar 1. Under agitation, the two complexes reached bar 2 and subsequently reacted with cDNA-POSS-PQDs, which were immobilized on MXene. Then, the encoded probes would be detached from bar 2 to generate FL signals in the supernatant. Notably, the pathogens can resume their free state and initiate next cycle. They swim between the two bars, and the FL signals can be gradually enhanced to maximum after several cycles. The FL signals from released encoded probes can be used to detect the analytes. In particular, live pathogens can be distinguished from dead ones by using an assay. The detection limits and linear range for S.T. and V.P. were 30 and 10 CFU/mL and 10²–10⁶ CFU/mL, respectively. Therefore, this assay has broad application potential for simultaneous on-site detection of various live pathogenic bacteria in water.
- Aptasensor,
- Live bacteria,
- Multiple detection,
- Multicolor perovskite quantum dots,
- Dual-stirring-bar

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References(39)

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