Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

Zihan Zhao; Yue Liu; Yushi Zhang; Zeyu Geng; Rina Su; Lipeng Zhou; Chao Han; Zhanjun Wang; Shuangcheng Ma; Weidong Li

doi:10.1016/j.jpha.2022.06.007

Volume 12 Issue 5

Nov. 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(5): 733-742

Zihan Zhao, Yue Liu, Yushi Zhang, Zeyu Geng, Rina Su, Lipeng Zhou, Chao Han, Zhanjun Wang, Shuangcheng Ma, Weidong Li. Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 733-742. doi: 10.1016/j.jpha.2022.06.007

Citation:

Zihan Zhao, Yue Liu, Yushi Zhang, Zeyu Geng, Rina Su, Lipeng Zhou, Chao Han, Zhanjun Wang, Shuangcheng Ma, Weidong Li. Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 733-742. doi: 10.1016/j.jpha.2022.06.007

Citation:

Zihan Zhao, Yue Liu, Yushi Zhang, Zeyu Geng, Rina Su, Lipeng Zhou, Chao Han, Zhanjun Wang, Shuangcheng Ma, Weidong Li. Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses[J]. Journal of Pharmaceutical Analysis, 2022, 12(5): 733-742. doi: 10.1016/j.jpha.2022.06.007

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Evaluation of the chemical profile from four germplasms sources of Pruni Semen using UHPLC-LTQ-Orbitrap-MS and multivariate analyses

doi: 10.1016/j.jpha.2022.06.007

a. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing, 102488, China;
b. Institute of Forestry and Grassland Ecology, Ningxia Academy of Agricultural and Forestry Sciences, Yinchuan, 750002, China;
c. Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, National Institutes for Food and Drug Control, Beijing, 100050, China

Funds:

This study was sponsored by the Key Research and Development Programs in the Ningxia Hui Autonomous Region, China (Grant No.: 2020BBF02027) and Beijing Natural Science Foundation (Grant No.: 5212014).

Received Date: Mar. 03, 2022
Accepted Date: Jun. 28, 2022
Rev Recd Date: Jun. 18, 2022
Publish Date: Jul. 06, 2022

Abstract

Abstract

Pruni Semen, the seed of several unique Prunus plants, is a traditional purgative herbal material. To determine the authentic sources of Pruni Semen, 46 samples from four species were collected and analyzed. Ten compounds including multiflorin A (Mul A), a notable purative compound, were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy. Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry, and acetyl flavonoid glycosides were recognized as characteristic constituents. The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel. Based on this, methods for identifying Pruni Semen from different sources were established using chemical fingerprinting, quantitative analysis of the eight principal compounds, hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The results showed that the samples were divided into two categories: one is the small seeds from Prunus humilis (Ph) and Prunus japonica (Pj), and the other is the big seeds from Prunus pedunculata (Pp) and Prunus triloba (Pt). The average content of Mul A was 3.02, 6.93, 0.40, and 0.29 mg/g, while the average content of amygdalin was 18.5, 17.7, 31.5, and 30.9 mg/g in Ph, Pj, Pp, and Pt, respectively. All the above information suggests that small seeds might be superior sources of Pruni Semen. This is the first comprehensive report on the identification of chemical components in Pruni Semen from different species.
- Pruni semen,
- Acetyl flavonoids,
- Amygdalin,
- Multivariate analyses,
- Authentic sources

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References(37)

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