Volume 12 Issue 4
Sep.  2022
Turn off MathJax
Article Contents
Nikita Looby, Anna Roszkowska, Aadil Ali, Barbara Bojko, Marcelo Cypel, Janusz Pawliszyn. Metabolomic fingerprinting of porcine lung tissue during pre-clinical prolonged ex vivo lung perfusion using in vivo SPME coupled with LC-HRMS[J]. Journal of Pharmaceutical Analysis, 2022, 12(4): 590-600. doi: 10.1016/j.jpha.2022.06.002
Citation: Nikita Looby, Anna Roszkowska, Aadil Ali, Barbara Bojko, Marcelo Cypel, Janusz Pawliszyn. Metabolomic fingerprinting of porcine lung tissue during pre-clinical prolonged ex vivo lung perfusion using in vivo SPME coupled with LC-HRMS[J]. Journal of Pharmaceutical Analysis, 2022, 12(4): 590-600. doi: 10.1016/j.jpha.2022.06.002

Metabolomic fingerprinting of porcine lung tissue during pre-clinical prolonged ex vivo lung perfusion using in vivo SPME coupled with LC-HRMS

doi: 10.1016/j.jpha.2022.06.002
Funds:

We would like to thank our collaborators at Millipore Sigma for providing us with the SPME fibers. We are grateful to the Canadian Institute of Health Research (CIHR) – Natural Sciences and Engineering Research Council (NSERC) of the Canada Collaborative Health Research Projects program for their financial support (Grant No.: 355935) and the Natural Sciences and Engineering Research Council of Canada Industrial Research Chair (IRC) program.

  • Received Date: Jan. 13, 2022
  • Accepted Date: Jun. 02, 2022
  • Rev Recd Date: May 28, 2022
  • Publish Date: Jun. 08, 2022
  • Normothermic ex vivo lung perfusion (NEVLP) has emerged as a modernized organ preservation technique that allows for detailed assessment of donor lung function prior to transplantation. The main goal of this study was to identify potential biomarkers of lung function and/or injury during a prolonged (19 h) NEVLP procedure using in vivo solid-phase microextraction (SPME) technology followed by liquid chromatography-high resolution mass spectrometry (LC-HRMS). The use of minimally invasive in vivo SPME fibers for repeated sampling of biological tissue permits the monitoring and evaluation of biochemical changes and alterations in the metabolomic profile of the lung. These in vivo SPME fibers were directly introduced into the lung and were also used to extract metabolites (on-site SPME) from fresh perfusate samples collected alongside lung samplings. A subsequent goal of the study was to assess the feasibility of SPME as an in vivo method in metabolomics studies, in comparison to the traditional in-lab metabolomics workflow. Several upregulated biochemical pathways involved in pro- and anti-inflammatory responses, as well as lipid metabolism, were observed during extended lung perfusion, especially between the 11th and 12th hours of the procedure, in both lung and perfusate samples. However, several unstable and/or short-lived metabolites, such as neuroprostanes, have been extracted from lung tissue in vivo using SPME fibers. On-site monitoring of the metabolomic profiles of both lung tissues through in vivo SPME and perfusate samples on site throughout the prolonged NEVLP procedure can be effectively performed using in vivo SPME technology.
  • loading
  • M.K. Hsin, R. Zamel, M. Cypel, et al., Metabolic profile of ex vivo lung perfusate yields biomarkers for lung transplant outcomes, Ann. Surg. 267 (2018) 196-197
    M.A. Roman, S. Nair, S. Tsui, et al., Ex vivo lung perfusion:a comprehensive review of the development and exploration of future trends, Transplantation. 96 (2013) 509-518
    M. Cypel, J.C. Yeung, S. Hirayama, et al., Technique for prolonged normothermic ex vivo lung perfusion, J. Heart Lung Transplant. 27 (2008) 1319-1325
    M. Cypel, M. Rubacha, J. Yeung, et al., Normothermic ex vivo perfusion prevents lung injury compared to extended cold preservation for transplantation, Am. J. Transplant. 9 (2009) 2262-2269
    M. Galasso, J.J. Feld, Y. Watanabe, et al., Inactivating hepatitis C virus in donor lungs using light therapies during normothermic ex vivo lung perfusion, Nat. Commun. 10 (2019), 481
    M. Cypel, J.C. Yeung, M. Liu, et al., Normothermic ex vivo lung perfusion in clinical lung transplantation, N. Engl. J. Med. 364 (2011) 1431-1440
    M. Kan, M. Shumyatcher, B.E. Himes, Using omics approaches to understand pulmonary diseases, Respir. Res. 18 (2017), 149
    I.V. Yang, D.A. Schwartz, Epigenetic control of gene expression in the lung, Am. J. Respir. Crit. Care Med. 183 (2011) 1295-1301
    O. Fiehn, Metabolomics:the link between genotypes and phenotypes, Plant Mol. Biol. 48 (2002) 155-171
    B. Bojko, K. Gorynski, G.A. Gomez-Rios, et al., Solid phase microextraction fills the gap in tissue sampling protocols, Anal. Chim. Acta 803 (2013) 75-81
    B. Bojko, K. Gorynski, G.A. Gomez-Rios, et al., Low invasive in vivo tissue sampling for monitoring biomarkers and drugs during surgery, Lab. Invest. 94 (2014) 586-594
    E. Cudjoe, B. Bojko, I. de Lannoy, et al., Solid-phase microextraction:a complementary in vivo sampling method to microdialysis, Angew Chem. Int. Ed. Engl. 52 (2013) 12124-12126
    E. Cudjoe, B. Bojko, P. Togunde, et al., In vivo solid-phase microextraction for tissue bioanalysis, Bioanalysis 4 (2012) 2605-2619
    N. Reyes-Garcés, M. Diwan, E. Boyaci, et al., In vivo brain sampling using a microextraction probe reveals metabolic changes in rodents after deep brain stimulation, Anal. Chem. 91 (2019) 9875-9884
    B. Bojko, N. Looby, M. Olkowicz, et al., Solid phase microextraction chemical biopsy tool for monitoring of doxorubicin residue during in vivo lung chemo-perfusion, J. Pharm. Anal. 11 (2021) 37-47
    D. Vuckovic, J. Pawliszyn, Systematic evaluation of solid-phase microextraction coatings for untargeted metabolomic profiling of biological fluids by liquid chromatography-mass spectrometry, Anal. Chem. 83 (2011) 1944-1954
    N. Reyes-Garcés, E. Gionfriddo, Recent developments and applications of solid phase microextraction as a sample preparation approach for mass-spectrometry-based metabolomics and lipidomics, Trac. Trends Anal. Chem. 113 (2019) 172-181
    G.A. Gómez-Ríos, M. Tascon, N. Reyes-Garcés, et al., Quantitative analysis of biofluid spots by coated blade spray mass spectrometry, a new approach to rapid screening, Sci. Rep. 7 (2017), 16104
    F.S. Mirnaghi, Y. Chen, L.M. Sidisky, et al., Optimization of the coating procedure for a high-throughput 96-blade solid phase microextraction system coupled with LC-MS/MS for analysis of complex samples, Anal. Chem. 83 (2011) 6018-6025
    M.C. Chambers, B. Maclean, R. Burke, et al., A cross-platform toolkit for mass spectrometry and proteomics, Nat. Biotechnol. 30 (2012) 918-920
    C.A. Smith, E.J. Want, G. O'Maille, et al., XCMS:processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification, Anal. Chem. 78 (2006) 779-787
    A. Roszkowska, M. Yu, V. Bessonneau, et al., Tissue storage affects lipidome profiling in comparison to in vivo microsampling approach, Sci. Rep. 8 (2018), 6980
    G. Libiseller, M. Dvorzak, U. Kleb, et al., IPO:a tool for automated optimization of XCMS parameters, BMC Bioinf. 16 (2015), 118
    M. Yu, M. Olkowicz, J. Pawliszyn, Structure/reaction directed analysis for LC-MS based untargeted analysis, Anal. Chim. Acta 1050 (2019) 16-24
    K. Uppal, D.I. Walker, D.P. Jones, xMSannotator:an R package for network-based annotation of high-resolution metabolomics data, Anal. Chem. 89 (2017) 1063-1067
    C. Gladine, J.-C. Laurie, C. Giulia, et al., Neuroprostanes, produced by free-radical mediated peroxidation of DHA, inhibit the inflammatory response of human macrophages, Free Radic. Biol. Med. 75 (2014), S15
    E. Miller, A. Morel, L. Saso, et al., Isoprostanes and neuroprostanes as biomarkers of oxidative stress in neurodegenerative diseases, Oxid. Med. Cell. Longev. 2014 (2014), 572491
    T. Murata, K. Aritake, Y. Tsubosaka, et al., Anti-inflammatory role of PGD2 in acute lung inflammation and therapeutic application of its signal enhancement, Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 5205-5210
    A. Ariel, C.N. Serhan, Resolvins and protectins in the termination program of acute inflammation, Trends Immunol. 28 (2007) 176-183
    M. Yu, S. Lendor, A. Roszkowska, et al., Metabolic profile of fish muscle tissue changes with sampling method, storage strategy and time, Anal. Chim. Acta 1136 (2020) 42-50
    B. Bojko, N. Reyes- Garces, V. Bessonneau, et al., Solid-phase microextraction in metabolomics, Trac. Trends Anal. Chem. 61 (2014) 168-180
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Figures(1)

    Article Metrics

    Article views (190) PDF downloads(19) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return