Modifying current thin-film microextraction (TFME) solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography

&#321;ukasz Sobczak; Dominika Ko&#322;odziej; Krzysztof Gory&#324;ski

doi:10.1016/j.jpha.2021.12.007

Volume 12 Issue 3

Jun. 2022

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Łukasz Sobczak, Dominika Kołodziej, Krzysztof Goryński. Modifying current thin-film microextraction (TFME) solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 470-480. doi: 10.1016/j.jpha.2021.12.007

Citation:

Łukasz Sobczak, Dominika Kołodziej, Krzysztof Goryński. Modifying current thin-film microextraction (TFME) solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 470-480. doi: 10.1016/j.jpha.2021.12.007

Citation:

Łukasz Sobczak, Dominika Kołodziej, Krzysztof Goryński. Modifying current thin-film microextraction (TFME) solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 470-480. doi: 10.1016/j.jpha.2021.12.007

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Modifying current thin-film microextraction (TFME) solutions for analyzing prohibited substances:Evaluating new coatings using liquid chromatography

doi: 10.1016/j.jpha.2021.12.007

Bioanalysis Scientific Group, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz at Nicolaus Copernicus University in Toruń, 85-089, Bydgoszcz, Poland

Funds:

and Arkadia Ciepłuch (M.Sc.) and Marcin Stachowiak (M.Sc.) for their help during preparation of the TFME blades.

Shim-Pol A.M. Borzymowski for technical assistance

Permission No.: WIFBY-KK.857.2.4.2016).The authors would like to thank the Department of Pharmacodynamics and Molecular Pharmacology, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz at Nicolaus Copernicus University in Toruń, Poland, for the access to the Shimadzu LCMS-8060 instrument

Department of Medicinal Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz at Nicolaus Copernicus University in Toruń, Poland, for access to the Shimadzu LCMS-8045 instrument and CentriVap Concentrator

This study was supported by the National Centre for Research and Development under the Lider IX programme (Grant No.: LIDER/44/0164/L-9/17/NCBR/2018). Permission to conduct experiments with controlled substances was issued by the local Pharmaceutical Inspector (Kujawsko-Pomorski Wojewódzki Inspektor Farmaceutyczny w Bydgoszczy

Received Date: May 24, 2021
Accepted Date: Dec. 31, 2021
Rev Recd Date: Dec. 30, 2021
Publish Date: Jan. 04, 2022

Abstract

Abstract

For identifying and quantifying prohibited substances, solid-phase microextraction (SPME) continues to arouse interest as a sample preparation method. However, the practical implementation of this method in routine laboratory testing is currently hindered by the limited number of coatings compatible with the ubiquitous high-performance liquid chromatography (HPLC) systems. Only octadecyl (C₁₈) and polydimethylsiloxane/divinylbenzene ligands are currently marketed for this purpose. To address this situation, the present study evaluated 12 HPLC-compatible coatings, including several chemistries not currently used in this application. The stationary phases of SPME devices in the geometry of thin film-coated blades were prepared by applying silica particles bonded with various functional ligands (C₁₈, octyl, phenyl-hexyl, 3-cyanopropyl, benzenesulfonic acid, and selected combinations of these), as well as unbonded silica, to a metal support. Most of these chemistries have not been previously used as microextraction coatings. The 48 most commonly misused substances were selected to assess the extraction efficacy of each coating, and eight desorption solvent compositions were used to optimize the desorption conditions. All samples were analyzed using an HPLC system coupled with triple quadrupole tandem mass spectrometry. This evaluation enables selection of the best-performing coatings for quantifying prohibited substances and investigates the relationship between extraction efficacy and the physicochemical characteristics of the analytes. Ultimately, using the most suitable coatings is essential for trace-level analysis of chemically diverse prohibited substances.
- Sample preparation,
- Solid-phase microextraction,
- Thin-film microextraction,
- Prohibited substances,
- Drugs of abuse,
- High-performance liquid chromatography

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References(53)

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