Potential use of a dried saliva spot (DSS) in therapeutic drug monitoring and disease diagnosis

Yu Han; Xi-Ling Li; Minghui Zhang; Jing Wang; Su Zeng; Jun Zhe Min

doi:10.1016/j.jpha.2021.11.001

Volume 12 Issue 6

Dec. 2022

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Yu Han, Xi-Ling Li, Minghui Zhang, Jing Wang, Su Zeng, Jun Zhe Min. Potential use of a dried saliva spot (DSS) in therapeutic drug monitoring and disease diagnosis[J]. Journal of Pharmaceutical Analysis, 2022, 12(6): 815-823. doi: 10.1016/j.jpha.2021.11.001

Citation:

Yu Han, Xi-Ling Li, Minghui Zhang, Jing Wang, Su Zeng, Jun Zhe Min. Potential use of a dried saliva spot (DSS) in therapeutic drug monitoring and disease diagnosis[J]. Journal of Pharmaceutical Analysis, 2022, 12(6): 815-823. doi: 10.1016/j.jpha.2021.11.001

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Potential use of a dried saliva spot (DSS) in therapeutic drug monitoring and disease diagnosis

doi: 10.1016/j.jpha.2021.11.001

Yu Han^a,
Xi-Ling Li^a,
Minghui Zhang^a,
Jing Wang^a,
Su Zeng^{b
,
,},
Jun Zhe Min^{a
,
,}

a. Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, Department of Pharmaceutical Analysis, College of Pharmacy, Yanbian University, Yanji, Jilin, 133002, China;
b. Institute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou, 310058, China

Funds:

This study has been supported by the National Natural Science Foundation of China (Grant Nos.: 82173782 and 32160234) and the Science and Technology Development Project, Education Department of Jilin Province of China (Grant No.: JJKH20191151KJ).

Received Date: Jun. 03, 2021
Accepted Date: Nov. 03, 2021
Rev Recd Date: Oct. 28, 2021
Publish Date: Dec. 26, 2022

Abstract

Abstract

In recent years, scientific researchers have increasingly become interested in noninvasive sampling methods for therapeutic drug monitoring and disease diagnosis. As a result, dried saliva spot (DSS), which is a sampling technique for collecting dried saliva samples, has been widely used as an alternative matrix to serum for the detection of target molecules. Coupling the DSS method with a highly sensitive detection instrument improves the efficiency of the preparation and analysis of biological samples. Furthermore, dried blood spots, dried plasma spots, and dried matrix spots, which are similar to those of the DSS method, are discussed. Compared with alternative biological fluids used in dried spot methods, including serum, tears, urine, and plasma, saliva has the advantage of convenience in terms of sample collection from children or persons with disabilities. This review aims to provide integral strategies and guidelines for dried spot methods to analyze biological samples by illustrating several dried spot methods. Herein, we summarize recent advancements in DSS methods from June 2014 to March 2021 and discuss the advantages and disadvantages of the key aspects of this method, including sample preparation and method validation. Finally, we outline the challenges and prospects of such methods in practical applications.
- Human saliva,
- Dried saliva spot,
- Therapeutic drug monitoring,
- Disease diagnosis

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References(83)

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