Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives

Yingai Sun; Yuqi Gao; Chunchao Tang; Gaopan Dong; Pei Zhao; Dunquan Peng; Tiantian Wang; Lupei Du; Minyong Li

doi:10.1016/j.jpha.2021.10.001

Volume 12 Issue 3

Jun. 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(3): 446-452

Yingai Sun, Yuqi Gao, Chunchao Tang, Gaopan Dong, Pei Zhao, Dunquan Peng, Tiantian Wang, Lupei Du, Minyong Li. Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 446-452. doi: 10.1016/j.jpha.2021.10.001

Citation:

Yingai Sun, Yuqi Gao, Chunchao Tang, Gaopan Dong, Pei Zhao, Dunquan Peng, Tiantian Wang, Lupei Du, Minyong Li. Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 446-452. doi: 10.1016/j.jpha.2021.10.001

Citation:

Yingai Sun, Yuqi Gao, Chunchao Tang, Gaopan Dong, Pei Zhao, Dunquan Peng, Tiantian Wang, Lupei Du, Minyong Li. Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 446-452. doi: 10.1016/j.jpha.2021.10.001

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Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives

doi: 10.1016/j.jpha.2021.10.001

a. Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, 250012, China;
b. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China;
c. State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China

Funds:

The present work was supported by grants from the National Natural Science Foundation of China (Grant Nos.: 81673393 and 81874308), Taishan Scholar Program in Shandong Province, and Shandong Natural Science Foundation (Grant No.: ZR2018ZC0233).

Received Date: Dec. 26, 2020
Accepted Date: Oct. 08, 2021
Rev Recd Date: Sep. 21, 2021
Publish Date: Oct. 09, 2021

Abstract

Abstract

In recent years, various methods for detecting exogenous and endogenous hypochlorite have been studied, considering its essential role as a biomolecule. However, the existing technologies still pose obstacles such as their invasiveness, high costs, and complicated operation. In the current study, we developed a glow-type chemiluminescent probe, hypochlorite chemiluminescence probe (HCCL)-1, based on the scaffold of Schaap's 1,2-dioxetane luminophores. To better explore the physiological and pathological functions of hypochlorite, we modified the luminophore scaffold of HCCL-1 to develop several probes, including HCCL-2, HCCL-3, and HCCL-4, which amplify the response signal of hypochlorite. By comparing the luminescent intensities of the four probes using the IVIS^® system, we determined that HCCL-2 with a limit of detection of 0.166 μM has enhanced sensitivity and selectivity for tracking hypochlorite both in vitro and in vivo.
- Glow-type,
- Chemiluminescent probe,
- 1,2-Dioxetane,
- Hypochlorite

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References(46)

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