Hyphenated LC-ABTS<sup>&#183;+</sup> and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in <i>Astragalus emarginatus</i> Labill. Extracts

Abir Chamandy; Minjie Zhao; Hassan Rammal; Sa&#239;d Ennahar

doi:10.1016/j.jpha.2021.09.008

Volume 12 Issue 2

May 2022

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Abir Chamandy, Minjie Zhao, Hassan Rammal, Saïd Ennahar. Hyphenated LC-ABTS·+ and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill. Extracts[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 253-262. doi: 10.1016/j.jpha.2021.09.008

Citation:

Abir Chamandy, Minjie Zhao, Hassan Rammal, Saïd Ennahar. Hyphenated LC-ABTS^·+ and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill. Extracts[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 253-262. doi: 10.1016/j.jpha.2021.09.008

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Hyphenated LC-ABTS^·+ and LC-DAD-HRMS for simultaneous analysis and identification of antioxidant compounds in Astragalus emarginatus Labill. Extracts

doi: 10.1016/j.jpha.2021.09.008

a. Analytical Chemistry of Bioactive Molecules and Pharmacognosy Team (CAMBAP), Hubert Curien Multidisciplinary Institute (IPHC), National Center for Scientific Research (CNRS), Faculty of Pharmacy, University of Strasbourg, Illkirch, 67400, France;
b. Research Platform of Analysis and Environmental Science (PRASE), Lebanese University, Hadath, 005, Lebanon;
c. Faculty of Agronomy, Lebanese University, Dekwaneh, 005, Lebanon

Funds:

(CNRS, Lebanon) for the identification of the plant specimens and to the Shouf Biosphere Reserve for granting permission for plant collection and facilitating the research work within the reserve.

The authors are grateful to Professor Georges Tohmé

Received Date: Mar. 15, 2021
Accepted Date: Sep. 15, 2021
Rev Recd Date: Aug. 30, 2021
Publish Date: Sep. 16, 2021

Abstract

Abstract

The compounds in leaf and stem extracts of Astragalus emarginatus Labill. (AEL), a plant species used in traditional Lebanese medicine, were investigated for antioxidant properties. First, the activity of various extracts was assessed using the Trolox equivalent antioxidant capacity, oxygen radical absorption capacity, and 2,2-diphenyl-1-picryl-hydrazyl-hydrate assays. The extract obtained using 30% ethanol showed the greatest activity. The antioxidant compounds in this extract were screened using a hyphenated high-performance liquid chromatography-2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) radical (ABTS^·+) system before being separated by ultra-high-performance liquid chromatography and identified using high-resolution mass spectrometry and ultra-violet-visible diode array detection. Approximately 40 compounds were identified. Hydroxycinnamates (caffeic, ferulic, and p-coumaric acid derivatives) and flavonoids (quercetin, luteolin, apigenin, and isorhamnetin derivatives) were the two main categories of the identified compounds. The active compounds were identified as caffeic acid derivatives and quercetin glycosides. In addition, the catechol moiety was shown to be key to antioxidant activity. This study showed that AEL is a source of natural antioxidants, which may explain its medicinal use.
- Antioxidant,
- Astragalus emarginatus,
- Catechol,
- Flavonoids,
- Hydroxycinnamates,
- Caffeic acid,
- Quercetin

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References(47)

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