Qualitative and quantitative evaluation <i>of Flos Puerariae</i> by using chemical fingerprint in combination with chemometrics method

Jing Han; Ke Xu; Quanxiang Yan; Wenwen Sui; Haotian Zhang; Sijie Wang; Zan Zhang; Ziyun Wei; Fei Han

doi:10.1016/j.jpha.2021.09.003

Volume 12 Issue 3

Jun. 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(3): 489-499

Jing Han, Ke Xu, Quanxiang Yan, Wenwen Sui, Haotian Zhang, Sijie Wang, Zan Zhang, Ziyun Wei, Fei Han. Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 489-499. doi: 10.1016/j.jpha.2021.09.003

Citation:

Jing Han, Ke Xu, Quanxiang Yan, Wenwen Sui, Haotian Zhang, Sijie Wang, Zan Zhang, Ziyun Wei, Fei Han. Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method[J]. Journal of Pharmaceutical Analysis, 2022, 12(3): 489-499. doi: 10.1016/j.jpha.2021.09.003

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Qualitative and quantitative evaluation of Flos Puerariae by using chemical fingerprint in combination with chemometrics method

doi: 10.1016/j.jpha.2021.09.003

a. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China;
b. Department of Ophthalmology, the Fourth People's Hospital of Shenyang, Shenyang, 110031, China;
c. Science and Technology Institute of Shenyang Open University, Shenyang, 110003, China;
d. Shenyang Harmony Health Medical Laboratory, Shenyang, 110016, China

Funds:

This work was supported by Liaoning Province Natural Science Foundation (Grant No.: 2021-MS-220).

Received Date: Mar. 23, 2021
Accepted Date: Sep. 11, 2021
Rev Recd Date: Aug. 29, 2021
Publish Date: Sep. 25, 2021

Abstract

Abstract

In order to better control the quality of Flos Puerariae (FP), qualitative and quantitative analyses were initially performed by using chemical fingerprint and chemometrics methods in this study. First, the fingerprint of FP was developed by HPLC and the chemical markers were screened out by similarity analysis (SA), hierarchical clustering analysis (HCA), principal components analysis (PCA), and orthogonal partial least squares discriminant analysis (OPLS-DA). Next, the chemical constituents in FP were profiled and identified by HPLC coupled to Fourier transform ion cyclotron resonance mass spectrometry (HPLC-FT-ICR MS). Then, the characteristic constituents in FP were quantitatively analyzed by HPLC. As a result, 31 common peaks were assigned in the fingerprint and 6 of them were considered as qualitative markers. A total of 35 chemical constituents were detected by HPLC-FT-ICR MS and 16 of them were unambiguously identified by comparing retention time, UV absorption wavelength, accurate mass, and MS/MS data with those of reference standards. Subsequently, the contents of glycitin, genistin, tectoridin, glycitein, genistein, and tectorigenin in 13 batches of FP were detected, ranging from 0.4438 to 11.06 mg/g, 0.955 to 1.726 mg/g, 9.81 to 57.22 mg/g, 3.349 to 41.60 mg/g, 0.3576 to 0.989 mg/g, and 2.126 to 9.99 mg/g, respectively. In conclusion, fingerprint analysis in combination with chemometrics methods could discover chemical markers for improving the quality control standard of FP. It is expected that the strategy applied in this study will be valuable for further quality control of other traditional Chinese medicines.
- Flos Puerariae,
- Fingerprint analysis,
- Chemometrics,
- HPLC-FT-ICR MS

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References(40)

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