Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography

Peijie Zhu; Weijia Chen; Qiqin Wang; Huihui Wu; Meng Ruan; Hongwu Wang; Zhengjin Jiang

doi:10.1016/j.jpha.2021.09.002

Volume 12 Issue 2

May 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(2): 332-338

Peijie Zhu, Weijia Chen, Qiqin Wang, Huihui Wu, Meng Ruan, Hongwu Wang, Zhengjin Jiang. Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 332-338. doi: 10.1016/j.jpha.2021.09.002

Citation:

Peijie Zhu, Weijia Chen, Qiqin Wang, Huihui Wu, Meng Ruan, Hongwu Wang, Zhengjin Jiang. Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 332-338. doi: 10.1016/j.jpha.2021.09.002

Citation:

Peijie Zhu, Weijia Chen, Qiqin Wang, Huihui Wu, Meng Ruan, Hongwu Wang, Zhengjin Jiang. Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 332-338. doi: 10.1016/j.jpha.2021.09.002

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Phosphatidylethanolamine functionalized biomimetic monolith for immobilized artificial membrane chromatography

doi: 10.1016/j.jpha.2021.09.002

Peijie Zhu^a,b,
Weijia Chen^b,c,
Qiqin Wang^b,d,
Huihui Wu^b,
Meng Ruan^b,
Hongwu Wang^{a
,
,},
Zhengjin Jiang^{b,d
,
,}

a. School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing, Guangdong, 526061, China;
b. Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou, 510632, China;
c. School of Pharmaceutical Engineering, Guangdong Food and Drug Vocational College, Guangzhou, 510632, China;
d. Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine & New Drug Research, Jinan University, Guangzhou, 510632, China

Funds:

This project was funded by the National Natural Science Foundation of China (Grant Nos.: 81872830 and 82073806), the Natural Science Foundation of Guangdong Province (Grant No.: 2020A1515010569), the Science and Technology Innovation Guidance Project of Zhaoqing City (Grant No.: 201804030103), and the Scientific Research Fund of Zhaoqing University (Grant No.: 201817).

Received Date: Jun. 02, 2020
Accepted Date: Sep. 05, 2021
Rev Recd Date: Jun. 30, 2021
Publish Date: Sep. 08, 2021

Abstract

Abstract

In this research, a new phospholipid based monolith was fabricated by in situ co-polymerization of 1-dodecanoyl-2-(11-methacrylamidoundecanoyl)-sn-glycero-3-phosphoethanolamine and ethylene dimethacrylate to mimick bio-membrane environment. Excellent physicochemical properties of this novel monolith that were achieved included column efficiency, stability, and permeability. Moreover, the biomimetic monolith showed outstanding separation capability for a series of intact proteins and small molecules. In particular, it exhibited good potential as an alternative to the commercial immobilized artificial membrane (IAM) column (IAM.PC.DD2) for studying drug-membrane interactions. This study not only enriched the types of IAM stationary phases, but also provided a simple model for the prediction of phosphatidylethanolamine related properties of drug candidates.
- Phosphatidylethanolamine,
- Biomimetic monolith,
- IAM,
- Drug-membrane interaction

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References(38)

References

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