Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from <i>Cordyceps militaris</i>

Jian Chen; Hai-Fang Li; Guozhu Zhao; Jin-Ming Lin; Xiangwei He

doi:10.1016/j.jpha.2021.05.003

Volume 11 Issue 4

Aug. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(4): 499-504

Jian Chen, Hai-Fang Li, Guozhu Zhao, Jin-Ming Lin, Xiangwei He. Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 499-504. doi: 10.1016/j.jpha.2021.05.003

Citation:

Jian Chen, Hai-Fang Li, Guozhu Zhao, Jin-Ming Lin, Xiangwei He. Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 499-504. doi: 10.1016/j.jpha.2021.05.003

Citation:

Jian Chen, Hai-Fang Li, Guozhu Zhao, Jin-Ming Lin, Xiangwei He. Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 499-504. doi: 10.1016/j.jpha.2021.05.003

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Matrix-assisted laser desorption ionization mass spectrometry based quantitative analysis of cordycepin from Cordyceps militaris

doi: 10.1016/j.jpha.2021.05.003

Jian Chen^a,b,
Hai-Fang Li^{b
,
,},
Guozhu Zhao^a,
Jin-Ming Lin^b,
Xiangwei He^{a
,
,}

a. Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Science and Technology, Beijing Forestry University, Beijing, 100083, China;
b. Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China

Funds:

This work was financially supported by Fundamental Research Funds for the Central Universities (Grant No. 2019ZY31), and the National Natural Science Foundation of China (Grant Nos. 21775086 and 31770110).

Received Date: Mar. 09, 2021
Accepted Date: May 27, 2021
Rev Recd Date: Apr. 29, 2021
Available Online: Jan. 24, 2022
Publish Date: Aug. 15, 2021

Abstract

Abstract

Cordycepin, which has great immunomodulatory activities such as anticancer, antifungal, antivirus, antileukemia and lipid-lowering ones, is the secondary metabolite of Cordyceps militaris (C. militaris). Liquid submerged fermentation is the common cultivation process to produce cordycepin. To optimize the fermentation process and improve production, monitoring the cordycepin secretion in the fermentation is essential. The measurement based on chromatography-mass spectrometry methods is generally involved in the complex sample pretreatments and time-consuming separation, so more rapid and convenient methods are required. Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is more attractive for faster and direct detection. Therefore, MALDI-MS detection combined with isotope-labeled internal standard was applied to the measurement of cordycepin content in the fermentation broth and mycelium. This method made accurate quantification of cordycepin in the range of 5–400 μg/mL with a relative standard deviation of 5.6%. The recovery rates of fermentation samples after the 1, 13, and 25 days were 90.15%, 94.27%, and 95.06%, respectively. The contents of cordycepin in the mycelium and fermentation broth were 136 mg/g and 148.39 mg/mL on the 20th culture day, respectively. The cordycepin secretion curve of the liquid fermentation of C. militaris was real-time traced over 25 days.
- Cordycepin,
- MALDI-MS,
- Isotope internal standard,
- Quantitative analysis,
- Liquid fermentation

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References(26)

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