<i>In-vitro</i> antioxidant and anti-inflammatory activities of ethanol stem-bark extract of <i>Blighia sapida</i> K.D. Koenig

Mukaila B. Adekola; Jacob O. Areola; Oladapo F. Fagbohun; Funke T. Asaolu; Gbenga E. Ogundepo; Adeniyi O. Fajobi; Olubunmi O. Babalola

doi:10.1016/j.jpha.2021.04.002

Volume 12 Issue 2

May 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(2): 350-354

Mukaila B. Adekola, Jacob O. Areola, Oladapo F. Fagbohun, Funke T. Asaolu, Gbenga E. Ogundepo, Adeniyi O. Fajobi, Olubunmi O. Babalola. In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 350-354. doi: 10.1016/j.jpha.2021.04.002

Citation:

Mukaila B. Adekola, Jacob O. Areola, Oladapo F. Fagbohun, Funke T. Asaolu, Gbenga E. Ogundepo, Adeniyi O. Fajobi, Olubunmi O. Babalola. In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 350-354. doi: 10.1016/j.jpha.2021.04.002

Citation:

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In-vitro antioxidant and anti-inflammatory activities of ethanol stem-bark extract of Blighia sapida K.D. Koenig

doi: 10.1016/j.jpha.2021.04.002

a. Department of Environmental Management and Toxicology, Federal University of Agriculture, Abeokuta, 110001, Nigeria;
b. Department of Medical Biochemistry, Obafemi Awolowo University, Ile-Ife, 230001, Nigeria;
c. Department of Biomedical Engineering, First Technical University Ibadan, Oyo State, Ibadan, 200001, Nigeria;
d. Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, Ile-Ife, 230001, Nigeria

Funds:

Our gratitude goes to Prof. O.O. Oyedapo, Department of Biochemistry and Molecular Biology, Obafemi Awolowo University, for having kindly provided all necessary information during the research.

Received Date: Jun. 29, 2020
Accepted Date: Apr. 06, 2021
Rev Recd Date: Apr. 04, 2021
Publish Date: Apr. 10, 2021

Abstract

Abstract

Blighia sapida (B. sapida) K.D. Koenig (Family Sapindaceae) is a branchless straight bole approximately 15 m in length. The study evaluated the antioxidant and anti-inflammatory activities of ethanol extract and fractions of B. sapida stem-bark using in vitro methods. Ethanol extract and its fractions were investigated for 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), total antioxidant capacity (TAC), and quantitative phenolic and flavonoid contents. Anti-inflammatory activity was evaluated using albumin denaturation and membrane stabilization assays. The extract and its fractions exhibited radical scavenging and anti-inflammatory properties. The ethyl acetate fraction possessed maximum phenolic and flavonoid contents (136.67 ±1.55 gallic acid equivalent mg/g and 75.76 ±4.03 quercetin equivalent mg/g, respectively). Antioxidant studies revealed that the ethyl acetate fraction displayed superior activity with an IC₅₀ = 0.09 ±0.03 mg/mL DPPH, and values of 146.96 ±3.81 ascorbic acid equivalent (AAE) mg/g and 359.20 ±4.98 AAE mg/g for FRAP and TAC, respectively. Furthermore, the anti-inflammatory activity was revealed by inhibition of heat-induced albumin denaturation and red blood cell membrane stabilization at concentrations of 200–1000 μg/mL and 50–250 μg/mL, respectively. The ethanol extract and fractions exhibited antioxidant and anti-inflammatory activities, with ethyl acetate fraction showing superior activity, which could be attributed to secondary metabolites, mainly phenolic compounds. Overall, the antioxidant and anti-inflammatory activities of B. sapida can be exploited by ethnomedicinal users.
- Blighia sapida,
- Extract,
- Anti-inflammatory,
- Antioxidant,
- Radical scavenging

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References(30)

References

D. Laveti, M. Kumar, R. Hemalatha, et al., Anti-Inflammatory Treatments for Chronic Diseases: A Review. Inflamm. Allergy Drug Targets. 12 (2013) 349-361

M. Allegra, Antioxidant and Anti-Inflammatory Properties of Plants Extract. Antioxidants. 8 (2019), 549

A. Sinmisola, M.B. Oluwasesan, A.P. Chukwuemeka, Blighia sapida K.D. Koenig: A review on its phytochemistry, pharmacological, and nutritional properties. J. Ethnopharmacol. 253 (2019) 446-459

PROTA4U, Plant Resources of Tropical Africa. https://www.prota4u.org/database (Accessed 12 November 2020)

O.A. Owolabi, D.B. James, A.B. Ibrahim, et al., Changes in Lipid Profile of Aqueous and Ethanolic Extract of Blighia sapida in Rats. Asian J. Med. Sci. 2 (2010) 177-180

M.B. Adekola, J.O. Areola, N.O. Omisore, et al., Sub-chronic toxicity study of ethanol stem-bark extract of Blighia sapida (Sapindaceae) in Wistar rats. Heliyon. 6 (2020), e02801

O.E. Apalowo, M.B. Adekola, F.T. Asaolu, et al., GC-MS analysis and inhibitory effect of Kigelia africana Leaf extract and fractions on 5-lipoxygenase. J. Pharmacogn. Phytochem. 8 (2019) 625-628

N. Boussoualim, A. Baghiani, I. Krache, et al., Inhibitory effects of Anchusa azurea extracts on xanthine oxidase activity and its hypouricemic effects on mice. Int. J. Pharm. Sci. 7 (2015) 195-199

N. Benariba, R. Djaziri, W. Bellakhdar et al., “Phytochemical screening and free radical scavenging activity of Citrullus colocynthis seeds extracts,” Asian Pac. J. Trop. Biomed. 3 (2013) 35-40

S. Aouachria, S. Boumerfeg, A. Benslama, et al., Acute, sub-acute toxicity and antioxidant activities (in vitro and in vivo) of Reichardia picroide crude extract. J. Ethnopharmacol. 208 (2017) 105-116

O. Amri, A. Zekhnini, A. Bouhaimi, et al., Anti-inflammatory activity of methanolic extract from Pistacia atlantica Desf. leaves. Pharmacog. J. 10 (2018) 71-76

N. Jahan, M.S. Parvin, N. Das, et al., Studies on the antioxidant activity of ethanol extract and its fractions from Pterygota alata leaves. J. Acute Med. 4 (2014) 103-108

O.O. Oyedapo, B.A. Akinpelu, K.F. Akinwumi, et al., Red Blood Cell Membrane Stabilizing Potential of Extracts of Lantana camara and its Fractions. Int. J. Plant Physiol. Biochem. 2 (2010) 46-51

S. Sakat, A.R. Juvekar, M.N. Gambhire, In vitro Antioxidant and Anti-Inflammatory Activity of Methanol Extract of Oxalis corniculata Linn. Int. J. Pharm. Pharm. Sci. 2 (2010) 146-155

N. Amraoui, N. Mayouf, N. Charef, et al., Antioxidant, anti-inflammatory and anti-arthritic activities of methanol extract of Tamus communis L. roots. Trop. J. Pharm. Res. 18 (2019) 1499-1506

C.O. Emmanuel, O.A. Helmina, C.E. Egwim, Phytochemical Constituents of Seeds of Ripe and Unripe Blighia sapida (Koenig) and Physicochemical Properties of the Seed Oil. Int. J. Pharm. Sci. Invent. 3 (2014) 31-40

K. Anurag, Y. Anumalik, G. Nitika, et al., Metabolites in Plants and its Classification. World. J. Pharm. Pharm. Sci. 4 (2015) 287-305

S. Senhaji, F. Lamchouri, H. Toufik, Phytochemical Content, Antibacterial, and Antioxidant Potential of Endemic Plant Anabasis aretioides Coss. & Moq. (Chenopodiaceae). Biomed. Res. Int. (2020) 1-16

K. Pavithra, S. Vadivukkarasi, Evaluation of Free Radical Scavenging Activity of Various Extracts of Leaves from Kedrostis foetidissima (Jacq.) Cogn. Food Science and Human Wellness. 4 (2015) 42-46

O.A. Ojo, B.O. Ajiboye, O.D. Imiere, et al., Antioxidative Properties of Blighia sapida K.D. Koenig Stem Bark Extract and Inhibitory Effects on Carbohydrate Hydrolyzing Enzymes Associated with Non-Insulin Dependent Diabetes Mellitus. Pharmacogn. J. 10 (2018) 376-383

J. Kobus-Cisowska, O. Szczepaniak, D. Szymanowska-Powalowska, et al., Antioxidant potential of various solvent extracts from Morus alba fruits and its major polyphenols composition. Cienc. Rural. 50 (2020) e20190371

O.A. Ojo, O.I. Oloyede, O.S. Tugbobo, et al., Antioxidant and inhibitory effect of scent leaf (Ocimum gratissimum) on Fe²⁺ and sodium nitroprusside induced lipid peroxidation in rat brain in vitro. Adv. Biol. Res. 8 (2014) 8-17

T. Riaz, M.A. Abbasi, M.I. Umar, et al., Comparison of Antioxidant Potential of Volatile Oils of Syzygium aromaticum and Illicium verum Relative to conventionally used Standards. J. Chem. Soc. Pak. 33 (2011) 200-204

P.O. Amira, H.O. Oloyede, Antioxidant Activity of Aqueous Extract of Blighia sapida Stem Bark in Alloxan-Induced Diabetic Rats. Glob. J. Med. Res. 17 (2017) 2249-4618

Alamgeer U.H. Hasan. Evaluation of in vitro and in vivo therapeutic efficacy of Ribes alpestre Decne in Rheumatoid arthritis. Braz. J. Pharm. Sci. 55 (2019), e17832

A. Meison, R. S. Muntadher, M. S. Ahmed, Detection about the Anti-Inflammatory Effect in Alcoholic Extract of Rosmarinus officinalis in vitro. J. Pharm. Sci. & Res. 11 (2019) 1531-1533

M.M. Musfiq, T. Ananthi, In vitro Anti-Inflammatory Activity of Seed Extract of Zea Mays (L.). Journal of Global Biosciences. 4 (2015) 2168-2173

Alamgeer, A.M. Uttra, U.H. Hasan, Anti-arthritic activity of aqueous-methanolic extract and various fractions of Berberis orthobotrys Bien ex Aitch. BMC Complement Altern. Med. 17 (2017) 1-16

S.S. Soares, E. Bekbolatova, M.D. Cotrim, et al., Chemistry and Pharmacology of the Kazakh Crataegus Almaatensis Pojark: An Asian Herbal Medicine. Antioxidants. 8 (2019), 300

M.B. Adekola, J.O. Areola, O.V. Oriyomi, et al., Brine Shrimps Lethality Test of Ethanol Extract and Gas Chromatography-Mass Spectrometry Analysis of Ethyl Acetate Fraction of Blighia sapida. Asian J. Pharm. Clin. Res. 13 (2020) 1-5

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