Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite

Yanan Yuan; Huanhuan Chen; Yehong Han; Fengxia Qiao; Hongyuan Yan

doi:10.1016/j.jpha.2021.03.013

Volume 12 Issue 2

May 2022

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Article Navigation > Journal of Pharmaceutical Analysis > 2022 > 12(2): 301-307

Yanan Yuan, Huanhuan Chen, Yehong Han, Fengxia Qiao, Hongyuan Yan. Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 301-307. doi: 10.1016/j.jpha.2021.03.013

Citation:

Yanan Yuan, Huanhuan Chen, Yehong Han, Fengxia Qiao, Hongyuan Yan. Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 301-307. doi: 10.1016/j.jpha.2021.03.013

Citation:

Yanan Yuan, Huanhuan Chen, Yehong Han, Fengxia Qiao, Hongyuan Yan. Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite[J]. Journal of Pharmaceutical Analysis, 2022, 12(2): 301-307. doi: 10.1016/j.jpha.2021.03.013

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Analysis of anticancer compound, indole-3-carbinol, in broccoli using a new ultrasound-assisted dispersive-filter extraction method based on poly(deep eutectic solvent)-graphene oxide nanocomposite

doi: 10.1016/j.jpha.2021.03.013

a. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, College of Pharmaceutical Science, Hebei University, Baoding, Hebei, 071002, China;
b. Key Laboratory of Public Health Safety of Hebei Province, Institute of Life Science and Green Development, College of Public Health, Hebei University, Baoding, Hebei, 071002, China;
c. College of Biochemistry, Baoding University, Baoding, Hebei, 071000, China

Funds:

This work is supported by the National Natural Science Foundation of China (Grant Nos.: 82073605 and 81803287), the Natural Science Foundation of Hebei Province (Grant Nos.: B2018201270 and H2021201002), the Talent Engineering Training Foundation of Hebei Province (Grant No.: A201802002), the Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (Grant No.: 2021174), Post-graduate's Innovation Fund Project of Hebei University (Grant No.: HBU2021ss006), and the Outstanding Doctoral Cultivation Project of Hebei University (Grant No.: YB201703).

Received Date: May 24, 2020
Accepted Date: Mar. 31, 2021
Rev Recd Date: Mar. 16, 2021
Publish Date: Apr. 07, 2021

Abstract

Abstract

Indole-3-carbinol (I3C), an important anticancer compound found in broccoli, has attracted considerable attention. The rapid extraction and accurate analysis of I3C in the pharmaceutical industry in broccoli is challenging as I3C is unstable at low pH and high temperature. In this study, a rapid, accurate, and low-cost ultrasound-assisted dispersive-filter extraction (UADFE) technique based on poly(deep eutectic solvent)-graphene oxide (PDES-GO) adsorbent was developed for the isolation and analysis of I3C in broccoli for the first time. PDES-GO with multiple adsorption interactions and a fast mass transfer rate was synthesized to accelerate adsorption and desorption. UADFE was developed by combining dispersive solid-phase extraction (DSPE) and filter solid-phase extraction (FSPE) to realize rapid extraction and separation. Based on the above two strategies, the proposed PDES-GO-UADFE method coupled with high-performance liquid chromatography (HPLC) allowed the rapid (15–16 min), accurate (84.3%–96.4%), and low-cost (adsorbent: 3.00 mg) analysis of I3C in broccoli and was superior to solid-phase extraction, DSPE, and FSPE methods. The proposed method showed remarkable linearity (r=0.9998; range: 0.0840–48.0 μg/g), low limit of quantification (0.0840 μg/g), and high precision (relative standard deviation ≤5.6%). Therefore, the PDES-GO-UADFE-HPLC method shows significant potential in the field of pharmaceutical analysis for the separation and analysis of anti-cancer compounds in complex plant samples.
- Indole-3-carbinol,
- Broccoli,
- Poly(deep eutectic solvent)-graphene oxide,
- Ultrasound-assisted dispersive-filter extraction,
- Rapid analysis

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References(40)

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