Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan

Zhanpeng Shang; Lulu Xu; Yi Kuang; Yan Lin; Shuang Liu; Long Sun; Tao Bo; Min Ye; Xue Qiao

doi:10.1016/j.jpha.2021.01.003

Volume 11 Issue 6

Dec. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(6): 717-725

Zhanpeng Shang, Lulu Xu, Yi Kuang, Yan Lin, Shuang Liu, Long Sun, Tao Bo, Min Ye, Xue Qiao. Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 717-725. doi: 10.1016/j.jpha.2021.01.003

Citation:

Zhanpeng Shang, Lulu Xu, Yi Kuang, Yan Lin, Shuang Liu, Long Sun, Tao Bo, Min Ye, Xue Qiao. Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 717-725. doi: 10.1016/j.jpha.2021.01.003

Citation:

Zhanpeng Shang, Lulu Xu, Yi Kuang, Yan Lin, Shuang Liu, Long Sun, Tao Bo, Min Ye, Xue Qiao. Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan[J]. Journal of Pharmaceutical Analysis, 2021, 11(6): 717-725. doi: 10.1016/j.jpha.2021.01.003

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Simultaneous determination of 35 constituents and elucidation of effective constituents in a multi-herb Chinese medicine formula Xiaoer-Feire-Kechuan

doi: 10.1016/j.jpha.2021.01.003

Zhanpeng Shang^a,
Lulu Xu^a,
Yi Kuang^a,
Yan Lin^b,
Shuang Liu^a,
Long Sun^c,
Tao Bo^c,
Min Ye^{a
,
,},
Xue Qiao^{a
,
,}

a. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China;
b. School of Pharmaceutical Sciences, Guizhou Medical University, Guizhou, 550025, China;
c. Thermo Fisher Scientific, Beijing, 100102, China

Funds:

The work was supported by the National Key Research and Development Program of China (Grant No.: 2018YFC1707304, 2018YFC1707301), Beijing Natural Science Foundation (Grant No.: JQ18027), and National Natural Science Foundation of China (Grant No.: 81725023). We thank Sunflower Pharmaceutical Co., Ltd. for providing the samples.

Received Date: Apr. 09, 2020
Accepted Date: Jan. 24, 2021
Rev Recd Date: Sep. 24, 2020
Available Online: Jan. 12, 2022
Publish Date: Dec. 15, 2021

Abstract

Abstract

Xiaoer-Feire-Kechuan (XFK) is an 11-herb Chinese medicine formula to treat cough and pulmonary inflammation. The complicated composition rendered its chemical analysis and effective-component elucidation. In this study, we combined quantitative analysis and bioactivity test to reveal the anti-inflammatory constituents of XFK. First, UPLC-DAD and UHPLC/Q-Orbitrap-MS methods were established and validated to quantify 35 analytes (covering 9 out of 11 herbs) in different XFK formulations. Parallel reaction monitoring mode built in Q-Orbitrap-MS was used to improve the sensitivity and selectivity. Then, anti-inflammatory activities of the 35 analytes were analyzed using in vitro COX-2 inhibition assay. Finally, major analytes forsythosides H, I, A (8–10), and baicalin (15) (total contents varied from 21.79 to 91.20 mg/dose in different formulations) with significant activities (inhibitory rate ≥ 80%) were proposed as the anti-inflammatory constituents of XFK. The present study provided an effective strategy to discover effective constituents of multi-herb formulas.
- Multi-herb formulas,
- Effective constituents,
- Xiaoer-Feire-Kechuan formula (XFK),
- Parallel reaction monitoring (PRM),
- Cyclooxygenase-2 (COX-2)

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References(24)

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