Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies

Sourav Das; Leader Langbang; Mahabul Haque; Vinay Kumar Belwal; Kripamoy Aguan; Atanu Singha Roy

doi:10.1016/j.jpha.2020.12.003

Volume 11 Issue 4

Aug. 2021

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Sourav Das, Leader Langbang, Mahabul Haque, Vinay Kumar Belwal, Kripamoy Aguan, Atanu Singha Roy. Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 422-434. doi: 10.1016/j.jpha.2020.12.003

Citation:

Sourav Das, Leader Langbang, Mahabul Haque, Vinay Kumar Belwal, Kripamoy Aguan, Atanu Singha Roy. Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 422-434. doi: 10.1016/j.jpha.2020.12.003

Citation:

Sourav Das, Leader Langbang, Mahabul Haque, Vinay Kumar Belwal, Kripamoy Aguan, Atanu Singha Roy. Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 422-434. doi: 10.1016/j.jpha.2020.12.003

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Biocompatible silver nanoparticles: An investigation into their protein binding efficacies, anti-bacterial effects and cell cytotoxicity studies

doi: 10.1016/j.jpha.2020.12.003

a. Department of Chemistry, National Institute of Technology Meghalaya, Shillong, 793003, India;
b. Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong, 793022, India;
c. Department of Bioscience and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, India

Funds:

Atanu Singha Roy acknowledges the Science and Engineering Research Board (ECR File No. ECR/2016/000159 and CRG File No. CRG/2019/000852), Government of India, for funding this work. The authors thank the Department of Bioscience and Bioengineering, IIT Guwahati for CD measurements. Sourav Das is indebted to TEQIP-III NIT Meghalaya and MH thanks NIT Meghalaya for fellowship. The authors acknowledge Sophisticated Analytical Instrumentation Centre, Tezpur University for P-XRD and Sophisticated Analytical Instrument Facility, North Eastern Hill University (Shillong) for the TEM and energy dispersive X-ray spectroscopy measurements, respectively. Sourav Das would like to thank Amanda N. Abraham, School of Science, RMIT University, Australia, for guidance on the determination of AgNP concentration in the colloidal phase using atomic absorption spectrometry.

Received Date: Apr. 07, 2020
Accepted Date: Dec. 01, 2020
Rev Recd Date: Nov. 27, 2020
Available Online: Jan. 24, 2022
Publish Date: Aug. 15, 2021

Abstract

Abstract

Green synthesis of silver nanoparticles (AgNPs) has garnered tremendous interest as conventional methods include the use and production of toxic chemicals, products, by-products and reagents. In this regard, the synthesis of AgNPs using green tea (GT) extract and two of its components, (−)-epigallocatechin gallate (EGCG) and (+)-catechin (Ct) as capping/stabilizing agents, is reported. The synthesized AgNPs showed antibacterial activity against the bacterial strains Staphylococcus aureus and Escherichia coli, along with anticancer activity against HeLa cells. After administering nanoparticles to the body, they come in contact with proteins and results in the formation of a protein corona; hence we studied the interactions of these biocompatible AgNPs with hen egg white lysozyme (HEWL) as a carrier protein. Static quenching mechanism was accountable for the quenching of HEWL fluorescence by the AgNPs. The binding constant (K_b) was found to be higher for EGCG-AgNPs ((2.309 ± 0.018) × 10⁴ M⁻¹) than for GT-AgNPs and Ct-AgNPs towards HEWL. EGCG-AgNPs increased the polarity near the binding site while Ct-AgNPs caused the opposite effect, but GT-AgNPs had no such observable effects. Circular dichroism studies indicated that the AgNPs had no such appreciable impact on the secondary structure of HEWL. The key findings of this research included the synthesis of AgNPs using GT extract and its constituent polyphenols, and showed significant antibacterial, anticancer and protein-binding properties. The –OH groups of the polyphenols drive the in situ capping/stabilization of the AgNPs during synthesis, which might offer new opportunities having implications for nanomedicine and nanodiagnostics.
- Green tea,
- Polyphenols,
- Silver nanoparticles,
- Anti-bacterial,
- Cytotoxicity,
- Hen egg white lysozyme (HEWL)

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References(83)

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