UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine

Xin Wang; Yue Du; Cuiting Wu; Ming Xu; Youping Liu; Xin Di

doi:10.1016/j.jpha.2020.09.001

Volume 11 Issue 4

Aug. 2021

Turn off MathJax

Article Contents

Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(4): 458-464

Xin Wang, Yue Du, Cuiting Wu, Ming Xu, Youping Liu, Xin Di. UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 458-464. doi: 10.1016/j.jpha.2020.09.001

Citation:

Xin Wang, Yue Du, Cuiting Wu, Ming Xu, Youping Liu, Xin Di. UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 458-464. doi: 10.1016/j.jpha.2020.09.001

Citation:

Xin Wang, Yue Du, Cuiting Wu, Ming Xu, Youping Liu, Xin Di. UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 458-464. doi: 10.1016/j.jpha.2020.09.001

PDF( 904 KB)

UHPLC-MS/MS analysis of cAMP and cGMP in rat plasma as potential biomarkers of Yin-Yang disharmony in traditional Chinese medicine

doi: 10.1016/j.jpha.2020.09.001

Xin Wang^a,
Yue Du^a,
Cuiting Wu^a,
Ming Xu^b,
Youping Liu^a,
Xin Di^{a
,
,}

a. Laboratory of Drug Metabolism and Pharmacokinetics, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, PR China;
b. Shenyang Analytical Application Center, Shimadzu (China) Co. Ltd., Shenyang, 167 Qingnian Street, Shenyang, 110016, PR China

Funds:

The authors acknowledge Prof. Jiuzhi Yuan from Shenyang Pharmaceutical University for the identification of the plant materials. This work was financially supported by the National Natural Science Foundation of China (No. J1103606).

Received Date: Feb. 20, 2020
Accepted Date: Sep. 01, 2020
Rev Recd Date: Jul. 10, 2020
Available Online: Jan. 24, 2022
Publish Date: Aug. 15, 2021

Abstract

Abstract

Cyclic 3′,5′-adenosine monophosphate (cAMP) and cyclic 3′,5′-guanosine monophosphate (cGMP) are considered as potential biomarkers for Yin-Yang disharmony in traditional Chinese medicine. However, phosphodiesterase-mediated ex vivo degradation of these molecules in biological samples may result in their underestimation. In the present study, a ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method was developed for determination of cAMP and cGMP in rat plasma, with special consideration of their stability ex vivo. Following precipitation of proteins from plasma samples with 0.4 M perchloric acid, the analytes were chromatographed on a Shimadzu Shim-pack-XR-ODS II column with 2.5 mM ammonium acetate and methanol in gradient mode. The MS/MS detection was performed using multiple reaction monitoring in the positive electrospray ionization mode. The lower limit of quantification was 0.27 ng/mL for cAMP and 0.37 ng/mL for cGMP. The method was used to determine the plasma cAMP and cGMP levels in normal and Yin deficiency diabetic rats treated with or without Rehmannia glutinosa. The developed method may be useful for evaluating the regulatory effects of Chinese herbal medicine on the levels of cAMP and cGMP in the body.
- cAMP,
- cGMP,
- Plasma,
- UHPLC-MS/MS,
- Yin-Yang disharmony

FullText(HTML)

References(27)

References

T. Haahr, J.S. Jensen, P. Humaidan, Research and business-The Yin and Yang in modern medicine, Reprod. Biomed. Online. 40 (2020) 613-616

Q.N. Hu, T. Yu, J.H. L, et al., End-to-end syndrome differentiation of Yin deficiency and Yang deficiency in traditional Chinese medicine, Comput. Methods Programs Biomed. 174 (2019) 9-15

C. Yan, Z. Luo, W. Li, et al., Disturbed Yin-Yang balance: Stress increases the susceptibility to primary and recurrent infections of herpes simplex virus type 1, Acta Pharm. Sin. B. 10 (2020) 383-398

G. Bianco, Fascial neuromodulation: an emerging concept linking acupuncture, fasciology, osteopathy and neuroscience, Eur. J. Transl. Myol. 29 (2019) 195-201

J.D. Adams, The effects of Yin, Yang and Qi in the skin on pain, Medicines (Basel). 3 (2016) 1-7

N.D. Goldberg, M.K. Haddox, S.E. Nicol, et al., Biologic regulation through opposing influences of cyclic GMP and cyclic AMP: the Yin Yang hypothesis, Adv. Cyclic Nucleotide Res. 5 (1975) 307-330

N.D. Goldberg, M.K. Haddox, C.E. Zeiling, et al., Cyclic GMP, cyclic AMP, and the yin yang hypothesis of biologic regulation, J. Invest. Dermatol. 67 (1976) 641-645

P. Schuhmacher, A. Wallang, Central blood pressure effects of guanylyl-imido-diphosphate and cyclic guanosine-monophosphate, Naunyn Schmiedebergs Arch. Pharmacol. 312 (1980) 31-35

K. Seki, M. Chisaka, M. Eriguchi, et al., An attempt to integrate Western and Chinese medicine: rationale for applying Chinese medicine as chronotherapy against cancer, Biomed. Pharmacother. 59 (2005) S132-S140

Q. Wang, X.J. Ren, S.L. Yao, et al., Clinical observation on the endocrinal and immune functions in subjects with yin-deficiency constitution, Chin. J. Integr. Med. 16 (2010) 28-32

Y. Tan, X.R. Liu, C. Lu, et al., Metabolic profiling reveals therapeutic biomarkers of processed Aconitum Carmichaeli Debx in treating hydrocortisone induced Kidney-Yang deficiency syndrome rats, J. Ethnopharmacol. 152 (2014) 585-593

P.Y. Gong, Y.W. He, J. Qi, et al., Synergistic nourishing 'Yin' effect of iridoid and phenylpropanoid glycosides from Radix Scrophulariae in vivo and in vitro, J. Ethnopharmacol. 246 (2020) 1-8

E. Engvall, K. Jonsson, P. Perlmann, Enzyme-linked immunosorbent assay. II. Quantitative assay of protein antigen, immunoglobulin G, by means of enzyme-labelled antigen and antibody-coated tubes, Biochim. Biophys. Acta. 251 (1971) 427-434

P. Pradelled, J. Grassi, D. Chabardes, et al., Enzyme immunoassays of adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate using acetylcholinesterase, Anal. Chem. 61 (1989) 447-453

M. Tsugawa, K. Moriwaki, S. Iida, et al., An enzyme-linked immunosorbent assay (ELISA) for guanosine 3',5'-cyclic monophosphate (cGMP) in human plasma and urine using monoclonal antibody, J. Immunoassay. 12 (1991) 263-276

Y. Zhang, D. Dufield, J. Klover, et al., Development and validation of an LC-MS/MS method for quantification of cyclic guanosine 3',5'-monophosphate (cGMP) in clinical applications: A comparison with a EIA method, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 877 (2009) 513-520

X. Ren, C. Wang, L. Wu, et al., Bioactivity assay of porcine relaxin based on cAMP accumulation in THP-1 cells quantified by LC-MS/MS, J. Pharm. Biomed. Anal. 111 (2015) 320-323

W. Goutier, P.A. Spaans, M.A.W. Van der Neut, et al., Development and application of an LC-MS/MS method for measuring the effect of (partial) agonists on cAMP accumulation in vitro, J. Neurosci. Methods. 188 (2010) 24-31

J. Martens-Lobenhoffer, C. Dautz, S.M. Bode-Boger, Improved method for the determination of cyclic guanosine monophosphate (cGMP) in human plasma by LC-MS/MS, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 878 (2010) 487-491

T. Van Damme, Y. Zhang, F. Lynen, et al., Determination of cyclic guanosine and cyclic adenosine monophosphate (cGMP and cAMP) in human plasma and animal tissues by solid phase extraction on silica and liquid chromatography-triple quadrupole mass spectrometry, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 909 (2012) 14-21

P. Oeckl, B. Ferger, Simultaneous LC-MS/MS analysis of the biomarkers cAMP and cGMP in plasma, CSF and brain tissue, J. Neurosci. Methods. 203 (2012) 338-343

H. Bahre, V. Kaever, Measurement of 2',3'-cyclic nucleotides by liquid chromatography-tandem mass spectrometry in cells, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 964 (2014) 208-211

J. Chen, A. Tabatabaei, D. Zook, et al., A surrogate analyte-based liquid chromatography-tandem mass spectrometry method for the determination of endogenous cyclic nucleotides in rat brain, J. Pharm. Biomed. Anal. 146 (2017) 361-368

H.Y. Zhang, J. Zhou, L. Zhang, et al., Characteristics of blood glucose excursions in type 2 diabetes mellitus patients with three different Traditional Chinese Medicine syndromes, J. Tradit. Chin. Med. 35 (2015) 537-545

W. Zheng, G. Wang, Z. Zhang, et al., Research progress on classical traditional Chinese medicine formula Liuwei Dihuang pills in the treatment of type 2 diabetes, Biomed. Pharmacother., 121 (2020) 109564

W. Yan, J. Han, L.M. Huang, et al., Correlation of syndrome succession and variance of laboratory targets of type 2 diabetic disease syndrome combination rat models, Chin. J. Tradit. Chin. Med. Pharm. 28 (2013) 2726-2729

Y. Liu, M. Xie, Y. Zhang, Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome, Zhong Xi Yi Jie He Xue Bao. 10 (2012) 100-108

Relative Articles

Supplements(0)

Cited By

Proportional views