Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China

Yong Deng; Cunwu Chen; Lingxiao Chen; Bangxing Han; Shaoping Li; Jing Zhao

doi:10.1016/j.jpha.2020.08.013

Volume 11 Issue 3

Jun. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(3): 284-291

Yong Deng, Cunwu Chen, Lingxiao Chen, Bangxing Han, Shaoping Li, Jing Zhao. Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 284-291. doi: 10.1016/j.jpha.2020.08.013

Citation:

Yong Deng, Cunwu Chen, Lingxiao Chen, Bangxing Han, Shaoping Li, Jing Zhao. Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 284-291. doi: 10.1016/j.jpha.2020.08.013

Citation:

Yong Deng, Cunwu Chen, Lingxiao Chen, Bangxing Han, Shaoping Li, Jing Zhao. Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 284-291. doi: 10.1016/j.jpha.2020.08.013

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Fast saccharide mapping method for quality consistency evaluation of commercial xylooligosaccharides collected in China

doi: 10.1016/j.jpha.2020.08.013

a. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China;
b. Anhui Engineering Laboratory for Conservation and Sustainable Utilization of Traditional Chinese Medicine Resources, West Anhui University, Lu'an, Anhui, China

Funds:

D Program of China (2019YFC1711300), the Science and Technology Development Fund, Macau SAR (File no. 0075/2018/A2, 034/2017/A1 and 0017/2019/AKP), and the University of Macau (File no. MYRG2018-00083-ICMS, MYRG2019-00128-ICMS, CPG2020-00021-ICMS).

The research was partially funded by grants from the National Natural Science Foundation of China (No. 81673389), the National Key R&

Received Date: Mar. 27, 2020
Accepted Date: Aug. 31, 2020
Rev Recd Date: Apr. 30, 2020
Available Online: Jan. 24, 2022
Publish Date: Jun. 15, 2021

Abstract

Abstract

Due to the extensive use of xylooligosaccharides (XOS) as functional food ingredients, many inferior goods and even adulterants are generally found in the market, which may pose a health hazard to certain populations. Chromatography method such as high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) is traditionally applied for the quality analysis of XOS. However, it is time consuming due to the prolonged separation and pre- or post- derivatization procedure. In this study, a fast saccharide mapping method based on matrix-assisted laser desorption/time-of-flight mass spectrometry (MALDI-TOF-MS) was developed for the quality consistency analysis of 22 batches of XOS collected from different manufacturers in China. The time needed for saccharides analysis using MALDI-MS was less than 30 min for one plate, at least 6 times faster than that by the traditional HPTLC chromatography method. In addition, MALDI-MS possessed higher resolution for XOS with DP4-DP7 based on the difference of m/z, which is hardly separated using HPTLC. The results showed that XOS were present only in samples XY01-XY11, samples XY12-XY14 only consisted of hex oligosaccharides, and samples XY15-XY22 were free of oligosaccharides. These indicate that the quality consistency of XOS products in the China market was poor, which should be carefully investigated.
- Xylooligosaccharides,
- Saccharide mapping,
- MALDI-MS,
- Quality control

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References(39)

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