Determination of bioactive components in the fruits of <i>Cercis chinensis</i> Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses

Yuan Hong; Xiaoyan Liao; Zilin Chen

doi:10.1016/j.jpha.2020.07.010

Volume 11 Issue 4

Aug. 2021

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Yuan Hong, Xiaoyan Liao, Zilin Chen. Determination of bioactive components in the fruits of Cercis chinensis Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 465-471. doi: 10.1016/j.jpha.2020.07.010

Citation:

Yuan Hong, Xiaoyan Liao, Zilin Chen. Determination of bioactive components in the fruits of Cercis chinensis Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses[J]. Journal of Pharmaceutical Analysis, 2021, 11(4): 465-471. doi: 10.1016/j.jpha.2020.07.010

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Determination of bioactive components in the fruits of Cercis chinensis Bunge by HPLC-MS/MS and quality evaluation by principal components and hierarchical cluster analyses

doi: 10.1016/j.jpha.2020.07.010

Yuan Hong^a,b,
Xiaoyan Liao^a,b,
Zilin Chen^{a,b
,
,}

a. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals; School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China;
b. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100080, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos. 82073808, 81872828, and 81573384).

Received Date: Apr. 14, 2020
Accepted Date: Jul. 29, 2020
Rev Recd Date: Jun. 30, 2020
Available Online: Jan. 24, 2022
Publish Date: Aug. 15, 2021

Abstract

Abstract

The fruits of leguminous plants Cercis Chinensis Bunge are still overlooked although they have been reported to be antioxidative because of the limited information on the phytochemicals of C. chinensis fruits. A simple, rapid and sensitive HPLC-MS/MS method was developed for the identification and quantitation of the major bioactive components in C. chinensis fruits. Eighteen polyphenols were identified, which are first reported in C. chinensis fruits. Moreover, ten components were simultaneously quantified. The validated quantitative method was proved to be sensitive, reproducible and accurate. Then, it was applied to analyze batches of C. chinensis fruits from different phytomorph and areas. The principal components analysis (PCA) realized visualization and reduction of data set dimension while the hierarchical cluster analysis (HCA) indicated that the content of phenolic acids or all ten components might be used to differentiate C. chinensis fruits of different phytomorph.
- C. chinensis fruits,
- HPLC-MS/MS,
- Polyphenols,
- Principal components analysis,
- Hierarchical cluster analysis

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References(27)

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