Identification and quantification of the bioactive components in <i>Osmanthus fragrans</i> roots by HPLC-MS/MS

Xiaoyan Liao; Yuan Hong; Zilin Chen

doi:10.1016/j.jpha.2020.06.010

Volume 11 Issue 3

Jun. 2021

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Xiaoyan Liao, Yuan Hong, Zilin Chen. Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 299-307. doi: 10.1016/j.jpha.2020.06.010

Citation:

Xiaoyan Liao, Yuan Hong, Zilin Chen. Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS[J]. Journal of Pharmaceutical Analysis, 2021, 11(3): 299-307. doi: 10.1016/j.jpha.2020.06.010

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Identification and quantification of the bioactive components in Osmanthus fragrans roots by HPLC-MS/MS

doi: 10.1016/j.jpha.2020.06.010

Xiaoyan Liao^a,b,
Yuan Hong^a,b,
Zilin Chen^{a,b
,
,}

a. Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education; Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals; School of Pharmaceutical Sciences, Wuhan University, Wuhan, 430071, China;
b. State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100080, China

Funds:

This work was supported by the National Natural Science Foundation of China (Grant Nos. 81872828 and 81573384) and the Large-scale Instrument and Equipment Sharing Foundation of Wuhan University (LF20191065).

Received Date: Feb. 03, 2020
Accepted Date: Jun. 30, 2020
Rev Recd Date: Jun. 29, 2020
Available Online: Jan. 24, 2022
Publish Date: Jun. 15, 2021

Abstract

Abstract

The roots of O. fragrans are also a valuable resource in addition to its flowers and fruits. In this study, the HPLC-MS/MS method used for analyzing the chemical constituents in O. fragrans roots extract was developed, which showed high sensitivity for both qualitative and quantitative analyses. Thirty-two compounds were first discovered in O. fragrans roots, one compound of which was reported for the first time. The simultaneous determination method for acteoside, isoacteoside, oleuropein and phillyrin was validated to be sensitive and accurate. Then it was applied to determine the content of bioactive components in O. fragrans roots from different cultivars. The content of oleuropein and phillyrin in the twelve batches was relatively stable, while the content of acteoside and isoacteoside varied greatly. Moreover, the therapeutic material basis and mechanism of O. fragrans roots exerting its traditional pharmacodynamics were analyzed by network pharmacology. The results showed that O. fragrans roots might be effective for the treatment of inflammation, cardiovascular diseases, cancer, and rheumatoid arthritis, which is consistent with the traditional pharmacodynamics of O. fragrans roots. This work can provide an analytical method for the comprehensive development of O. fragrans roots.
- Osmanthus fragrans roots,
- HPLC-MS/MS,
- Identification,
- Quantitation,
- Bioactive constituents,
- Network pharmacology

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References(40)

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