Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert

Ting Wang; Feina Li; Qinpei Lu; Gang Wu; Zhongke Jiang; Shaowei Liu; Xugela Habden; Elizaveta A. Razumova; Ilya A. Osterman; Petr V. Sergiev; Olga A. Dontsova; Xinxin Hu; Xuefu You; Chenghang Sun

doi:10.1016/j.jpha.2020.06.004

Volume 11 Issue 2

Apr. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(2): 241-250

Ting Wang, Feina Li, Qinpei Lu, Gang Wu, Zhongke Jiang, Shaowei Liu, Xugela Habden, Elizaveta A. Razumova, Ilya A. Osterman, Petr V. Sergiev, Olga A. Dontsova, Xinxin Hu, Xuefu You, Chenghang Sun. Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert[J]. Journal of Pharmaceutical Analysis, 2021, 11(2): 241-250. doi: 10.1016/j.jpha.2020.06.004

Citation:

Ting Wang, Feina Li, Qinpei Lu, Gang Wu, Zhongke Jiang, Shaowei Liu, Xugela Habden, Elizaveta A. Razumova, Ilya A. Osterman, Petr V. Sergiev, Olga A. Dontsova, Xinxin Hu, Xuefu You, Chenghang Sun. Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert[J]. Journal of Pharmaceutical Analysis, 2021, 11(2): 241-250. doi: 10.1016/j.jpha.2020.06.004

Citation:

Ting Wang, Feina Li, Qinpei Lu, Gang Wu, Zhongke Jiang, Shaowei Liu, Xugela Habden, Elizaveta A. Razumova, Ilya A. Osterman, Petr V. Sergiev, Olga A. Dontsova, Xinxin Hu, Xuefu You, Chenghang Sun. Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert[J]. Journal of Pharmaceutical Analysis, 2021, 11(2): 241-250. doi: 10.1016/j.jpha.2020.06.004

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Diversity, novelty, antimicrobial activity, and new antibiotics of cultivable endophytic actinobacteria isolated from psammophytes collected from Taklamakan Desert

doi: 10.1016/j.jpha.2020.06.004

a. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China;
b. Beijing Key Laboratory of Antimicrobial Agents, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China;
c. College of Life Science, Xinjiang Normal University, Urumchi, 830054, China;
d. Lomonosov Moscow State University, Moscow, 119992, Russia;
e. Center of Life Sciences, Skolkovo Institute of Science and Technology, Moscow, 143025, Russia;
f. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 119992, Russia

Funds:

The authors are grateful to Dr. Zhanfeng Xia from the Tarim University, Xinjiang, China, for assistance in sampling at Taklamakan Desert, and Dr. Mohsin T. Cheema from University of the Punjab and Dr. Rongfeng Li from Johns Hopkins University for useful discussion. The research work was supported by CAMS Innovation Fund for Medical Sciences (Grant Nos. CAMS 2017-I2M-B&

R-08 and 2017-I2M-1-012), the PUMC Doctoral Innovation Fund Project (Grant No. 2018-1007-16), the Drug Innovation Major Project of China (Grant No. 2018ZX09711001-007-002), the Russian Foundation for Basic Research (Grant No. 20-54-53014), and the National Natural Science Foundation of China (Grant No. 82011530051).

Received Date: Dec. 04, 2019
Accepted Date: Jun. 26, 2020
Rev Recd Date: Jun. 24, 2020
Publish Date: Aug. 06, 2020

Abstract

Abstract

Three hundred and twenty endophytic actinobacterial strains were isolated from psammophytes collected from Taklamakan Desert and identified. Among them, three strains already had been identified as new species of two genera and sixteen isolates showed relatively low 16S rRNA similarities < 98.6% to validly described species. Seventy-five of the isolates were selected as representative strains to screen antibacterial activity and mechanism. Forty-seven strains showed antagonistic activity against at least one of the indicator bacteria. Two Streptomyces strains produced bioactive compounds inducing DNA damage, and two Streptomyces strains produced bioactive compounds with inhibitory activity on protein biosynthesis. Notably, the strain Streptomyces sp. 8P21H-1 that demonstrated both strong antibacterial activity and inhibitory activity on protein biosynthesis was prioritized for exploring new antibiotics. Under the strategy of integrating genetics-based discovery program and MS/MS-based molecular networking, two new streptogramin-type antibiotics, i.e., acetyl-griseoviridin and desulphurizing griseoviridin, along with known griseoviridin, were isolated from the culture broth of strain 8P21H-1. Their chemical structures were determined by HR-MS, and 1D and 2D NMR. Desulphurizing griseoviridin and griseoviridin exhibited antibacterial activities by inhibiting translation.
- Taklamakan desert,
- Endophytic actinobacteria,
- Novelty,
- Diversity,
- Streptogramin-type antibiotic

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References(42)

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