Comprehensive metabolic profiling of <i>Alismatis Rhizoma</i> triterpenes in rats based on characteristic ions and a triterpene database

Lu Wang; Sen Li; Jiaxin Li; Zhongzhe Cheng; Yulin Feng; Hui Ouyang; Zhifeng Du; Hongliang Jiang

doi:10.1016/j.jpha.2020.03.010

Volume 11 Issue 1

Feb. 2021

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Article Navigation > Journal of Pharmaceutical Analysis > 2021 > 11(1): 96-107

Lu Wang, Sen Li, Jiaxin Li, Zhongzhe Cheng, Yulin Feng, Hui Ouyang, Zhifeng Du, Hongliang Jiang. Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database[J]. Journal of Pharmaceutical Analysis, 2021, 11(1): 96-107. doi: 10.1016/j.jpha.2020.03.010

Citation:

Lu Wang, Sen Li, Jiaxin Li, Zhongzhe Cheng, Yulin Feng, Hui Ouyang, Zhifeng Du, Hongliang Jiang. Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database[J]. Journal of Pharmaceutical Analysis, 2021, 11(1): 96-107. doi: 10.1016/j.jpha.2020.03.010

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Comprehensive metabolic profiling of Alismatis Rhizoma triterpenes in rats based on characteristic ions and a triterpene database

doi: 10.1016/j.jpha.2020.03.010

a. Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, China;
b. Department of Pharmacy, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China;
c. Jiangxi University of Traditional Chinese Medicine, Nanchang, China;
d. School of Pharmacy, Weifang Medical University, Weifang, China

Funds:

This study was financially supported by the National Natural Science Foundation of China (Grant No. 81803717 and U1603104).

Received Date: Oct. 27, 2019
Accepted Date: Mar. 21, 2020
Rev Recd Date: Mar. 20, 2020
Available Online: Jan. 24, 2022
Publish Date: Feb. 15, 2021

Abstract

Abstract

Alismatis Rhizoma (AR) is widely used in Chinese medicine, and its major bioactive components, triterpenes, reportedly possess various pharmacological activities. Therefore, it is very important to study the metabolism of triterpenes in vivo. However, the metabolism of AR triterpene extract has not been comprehensively elucidated due to its complex chemical components and metabolic pathways. In this study, an ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry method, which was based on the characteristic ions from an established database of known triterpenes, was used to analyze the major metabolites in rats following the oral administration of Alismatis Rhizoma extracts (ARE). As a result, a total of 233 constituents, with 85 prototype compounds and 148 metabolites, were identified for the first time. Hydrogenation, oxidation, sulfate and glucuronidation conjugation were the major metabolic pathways for triterpenes in AR. In addition, the mutual in vivo transformation of known ARE triterpenes was discovered and confirmed for the first time. Those results provide comprehensive insights into the metabolism of AR in vivo, which will be useful for future studies on its pharmacodynamics and pharmacokinetics. Moreover, this established strategy may be useful in metabolic studies of similar compounds.
- Alismatis rhizoma,
- Triterpenes,
- Metabolites,
- HPLC-QTOF-MS/MS

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