Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS

Ting Liu; Ruiyun Li; Yue Cui; Zhiguo Yu; Yunli Zhao

doi:10.1016/j.jpha.2019.11.007

Volume 10 Issue 6

Dec. 2020

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Article Navigation > Journal of Pharmaceutical Analysis > 2020 > 10(6): 581-587

Ting Liu, Ruiyun Li, Yue Cui, Zhiguo Yu, Yunli Zhao. Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS[J]. Journal of Pharmaceutical Analysis, 2020, 10(6): 581-587. doi: 10.1016/j.jpha.2019.11.007

Citation:

Ting Liu, Ruiyun Li, Yue Cui, Zhiguo Yu, Yunli Zhao. Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS[J]. Journal of Pharmaceutical Analysis, 2020, 10(6): 581-587. doi: 10.1016/j.jpha.2019.11.007

Citation:

Ting Liu, Ruiyun Li, Yue Cui, Zhiguo Yu, Yunli Zhao. Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS[J]. Journal of Pharmaceutical Analysis, 2020, 10(6): 581-587. doi: 10.1016/j.jpha.2019.11.007

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Metabonomic analysis of plasma biochemical changes in pyrexia rats after treatment with Gegenqinlian decoction, aspirin and itraconazole by UHPLC-FT-ICR-MS

doi: 10.1016/j.jpha.2019.11.007

Ting Liu^a,b,
Ruiyun Li^a,
Yue Cui^a,
Zhiguo Yu^a,
Yunli Zhao^{a
,
,}

a. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, 110016, China;
b. School of Pharmacy, Shenyang Medical College, Shenyang, 110034, China

Funds:

Funding: This work was supported by the Natural Science Foundation of China (NO. 81573629).

Received Date: Jul. 15, 2019
Accepted Date: Nov. 26, 2019
Rev Recd Date: Nov. 19, 2019
Available Online: Jan. 24, 2022
Publish Date: Dec. 10, 2020

Abstract

Abstract

A metabonomic approach involving an ultrahigh-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) was used to investigate the changes in the endogenous metabolites in the plasma of rats with yeast-induced pyrexia treated with Gegenqinlian decoction (GQLD), aspirin and itraconazole. The differences in the small molecule profiles of treatment using traditional Chinese medicine, etiological treatment and symptomatic treatment were elucidated. Thirty-six plasma metabolites were identified or putatively identified, and the effects of the three medicines on the thirty-six metabolites were studied. Their metabolic pathways indicated that GQLD, aspirin and itraconazole ameliorated the rats with yeast-induced pyrexia predominantly by regulating the metabolisms of phospholipid, sphingolipid, fatty acid oxidation, fatty acid amides, amino acid and glycerolipid in vivo. The pharmacodynamics and metabonomic results showed that the three medicines exhibited the therapeutic effects on pyrexia by regulating the perturbations of multiple metabolisms. The study provided a scientific basis for an in-depth understanding of the therapeutic effects of GQLD, aspirin and itraconazole on rats with yeast-induced pyrexia.
- Gegenqinlian decoction,
- Aspirin,
- Itraconazole,
- Pyrexia,
- UHPLC-FT-ICR-MS,
- Metabonomic

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References(27)

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