Volume 5 Issue 4
Aug.  2015
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Manjunath D. Meti, Sharanappa T. Nandibewoor, Shrinivas D. Joshi, Uttam A. More, Shivamurti A. Chimatadar. Multi-spectroscopic investigation of the binding interaction of fosfomycin with bovine serum albumin☆[J]. Journal of Pharmaceutical Analysis, 2015, 5(4): 249-255. doi: 10.1016/j.jpha.2015.01.004
Citation: Manjunath D. Meti, Sharanappa T. Nandibewoor, Shrinivas D. Joshi, Uttam A. More, Shivamurti A. Chimatadar. Multi-spectroscopic investigation of the binding interaction of fosfomycin with bovine serum albumin☆[J]. Journal of Pharmaceutical Analysis, 2015, 5(4): 249-255. doi: 10.1016/j.jpha.2015.01.004

Multi-spectroscopic investigation of the binding interaction of fosfomycin with bovine serum albumin☆

doi: 10.1016/j.jpha.2015.01.004
  • Publish Date: Aug. 10, 2015
  • The interaction between fosfomycin (FOS) and bovine serum albumin (BSA) has been investigated effectively by multi-spectroscopic techniques under physiological pH 7.4. FOS quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites n and observed binding constant KA were measured by the fluorescence quenching method. The thermodynamic parameters △G0, △H0 and △S0 were calculated at different temperatures according to the van't Hoff equation. The site of binding of FOS in the protein was proposed to be Sudlow's site I based on displacement experiments using site markers viz. warfarin, ibuprofen and digitoxin. The distance r between the donor (BSA) and acceptor (FOS) molecules was obtained according to the F?rster theory. The effect of FOS on the conformation of BSA was analyzed using synchronous fluorescence spectra (SFS), circular dichroism (CD) and 3D fluorescence spectra. A molecular modeling study further confirmed the binding mode obtained by the experimental studies.
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