Volume 2 Issue 2
Apr.  2012
Turn off MathJax
Article Contents
Hidetoshi Arakawa, Shigeo Nakabayashi, Ken-ichi Ohno, Masako Maeda. New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA[J]. Journal of Pharmaceutical Analysis, 2012, 02(2): 156-159. doi: 10.1016/j.jpha.2012.01.004
Citation: Hidetoshi Arakawa, Shigeo Nakabayashi, Ken-ichi Ohno, Masako Maeda. New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA[J]. Journal of Pharmaceutical Analysis, 2012, 02(2): 156-159. doi: 10.1016/j.jpha.2012.01.004

New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA

doi: 10.1016/j.jpha.2012.01.004
  • Publish Date: Apr. 10, 2012
  • Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 nm.The measurable range of HRP was 1.0 × 10-18 to 1.0 × 10-15 mol/assay,with a detection limit of 1.0 × 10-18 tmol/assay.The coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (67) PDF downloads(0) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return