Patricia Severino, Heloisa Silva, Eliana B.Souto, Maria Helena A.Santana, Teresa Cristina T.Dalla Costa. Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC[J]. Journal of Pharmaceutical Analysis, 2012, 02(1): 29-34. doi: 10.1016/j.jpha.2011.10.006
Citation:
Patricia Severino, Heloisa Silva, Eliana B.Souto, Maria Helena A.Santana, Teresa Cristina T.Dalla Costa. Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC[J]. Journal of Pharmaceutical Analysis, 2012, 02(1): 29-34. doi: 10.1016/j.jpha.2011.10.006
Patricia Severino, Heloisa Silva, Eliana B.Souto, Maria Helena A.Santana, Teresa Cristina T.Dalla Costa. Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC[J]. Journal of Pharmaceutical Analysis, 2012, 02(1): 29-34. doi: 10.1016/j.jpha.2011.10.006
Citation:
Patricia Severino, Heloisa Silva, Eliana B.Souto, Maria Helena A.Santana, Teresa Cristina T.Dalla Costa. Analysis of in vivo absorption of didanosine tablets in male adult dogs by HPLC[J]. Journal of Pharmaceutical Analysis, 2012, 02(1): 29-34. doi: 10.1016/j.jpha.2011.10.006
Biotechnological Processes Development Laboratory, School of Chemical Engineering, State University of Campinas-UNICAMP,Campinas, SP, Brazil
School of Pharmacy, University of Rio Grande do Sul-UFRGS, Porto Alegre, Rio Grande do Sul 90610-000, Brazil
School of Agrarian Science and Veterinary, Sāo Paulo State University-UNESP, Jaboticabal, Sāo Paulo 14.884-900, Brazil
Faculty of Health Sciences, Fernando Pessoa University(FCS-UFP), Rua Carlos da Maia, 296, P-4200-150 Porto, Portugal
Institute of Biotechnology and Bioengineering, Centre of Genomics and Biotechnology, University of Trás-os-Montes and Alto Douro(CGB-UTAD/IBB), P.O. Box 1013, P-5001-801, Vila Real, Portugal
Biotechnological Processes Development Laboratory, School of Chemical Engineering, State University of Campinas-UNICAMP,Campinas, SP, Brazil
Funds:
the Research Foundation of the State of Sāo Paulo (FAPESP/Brazil)
National Council of Technological and Scientific Development (CNPq/Brazil)
Fundacāo para a Ciēncia e Tecnologia do Ministério da Ciēncia e Tecnologia,Portugal
Didanosine is an effective antiviral drug in untreated and antiretroviral therapy-experienced patients with Human Immunodeficiency Virus (HIV).An automated system using on-line solid extraction and High performance Liquid Chromatography (HPLC) with ultraviolet (UV) detection was developed and validated for pharmacokinetic analysis of didanosiae in dog plasma.Modifications were introduced on a previous methodology for simultaneous analysis of antiretroviral drugs in human plasma.Extraction was carried out on C18 cartridges,with high extraction yield as stationary phase,whereas mobile phase consisted of a mixture of 0.02 M potassium phosphate buffer,acetonitrile (KH2PO4:acetonitrile:96∶4,v/v) and 0.5% (w/v) of heptane sulphonic acid.The pH was adjusted to 6.5 with triethylamine.All samples and standard solutions were chromatographed at 28℃.For an isocratic run,the flux was 1.0 mL/min,detection was at 250nm and injected volume was 20μL.The method was selective and linear for concentrations between 50 and 5000 ng/mL.Drug stability data ranged from 96% to 98%,and limit of quantification was 25 ng/mL.Extraction yield was up to 95%.Drug stability in dog plasma was kept frozen at -20℃ for one month after thee freeze-thaw cycles,and for 24 h after processing in the auto sampler.Assay was successfully applied to measure didanosine concentrations in plasma dogs.
DownLoad: