Spectrophotometric method for the determination of ketoconazole based on amplification reactions

This paper describes a sensitive spectrophotometric method developed for determination of Ketoconazole (KC) in tablets based on amplification reactions.Ketoconazole was oxidized with periodate,resulting in formation of KC2+ and iodate ions.After masking the excess periodate with molybdate,the iodate was treated with iodide to release iodine.The liberated iodine was transformed to ICl2ˉ species and extracted as ion-pair with rhodamine 6G into toluene for spectrophotometric measurement at 535nm.A linear calibration graph was obtained between 0.2136μg/mL and 1.7088 μg/mL of Ketoconazole with a molar absorptivity of 5 × 105 mol.L-1 cmˉ 1.The procedure was successfully applied for the determination of ketoconazole in tablet formulation.