Journal of Pharmaceutical Analysis

DNA barcoding in herbal medicine: Retrospective and prospective

Shilin Chen, Xianmei Yin, Jianping Han, Wei Sun, Hui Yao, Jingyuan Song, Xiwen Li

2023, 13(5): 431-441. doi: 10.1016/j.jpha.2023.03.008

Abstract(550) HTML Full Text PDF(53) [Cited by] (47)

Abstract:
DNA barcoding has been widely used for herb identification in recent decades, enabling safety and innovation in the field of herbal medicine. In this article, we summarize recent progress in DNA barcoding for herbal medicine to provide ideas for the further development and application of this techno...

Benzodiazepines in complex biological matrices: Recent updates on pretreatment and detection methods

Yi-Xin Zhang, Yuan Zhang, Yu Bian, Ya-Jie Liu, Ai Ren, Yu Zhou, Du Shi, Xue-Song Feng

2023, 13(5): 442-462. doi: 10.1016/j.jpha.2023.03.007

Abstract(1091) HTML Full Text PDF(29) [Cited by] (12)

Abstract:
Benzodiazepines (BDZs) are used in clinics for anxiolysis, anticonvulsants, sedative hypnosis, and muscle relaxation. They have high consumptions worldwide because of their easy availability and potential addiction. They are often used for suicide or criminal practices such as abduction and drug-fac...

Ginsenoside Rk3 is a novel PI3K/AKT-targeting therapeutics agent that regulates autophagy and apoptosis in hepatocellular carcinoma

Linlin Qu, Yannan Liu, Jianjun Deng, Xiaoxuan Ma, Daidi Fan

2023, 13(5): 463-482. doi: 10.1016/j.jpha.2023.03.006

Abstract(737) HTML Full Text PDF(85) [Cited by] (37)

Abstract:
Hepatocellular carcinoma (HCC) is the third leading cause of cancer death worldwide. Ginsenoside Rk3, an important and rare saponin in heat-treated ginseng, is generated from Rg1 and has a smaller molecular weight. However, the anti-HCC efficacy and mechanisms of ginsenoside Rk3 have not yet been ch...

Spatiotemporal pharmacometabolomics based on ambient mass spectrometry imaging to evaluate the metabolism and hepatotoxicity of amiodarone in HepG2 spheroids

Limei Li, Qingce Zang, Xinzhu Li, Ying Zhu, Shanjing Wen, Jiuming He, Ruiping Zhang, Zeper Abliz

2023, 13(5): 483-493. doi: 10.1016/j.jpha.2023.04.007

Abstract(585) HTML Full Text PDF(49) [Cited by] (14)

Abstract:
Three-dimensional (3D) cell spheroid models combined with mass spectrometry imaging (MSI) enables innovative investigation of in vivo-like biological processes under different physiological and pathological conditions. Herein, airflow-assisted desorption electrospray ionization-MSI (AFADESI-MSI) was...

Host cell protein quantification workflow using optimized standards combined with data-independent acquisition mass spectrometry

Steve Hessmann, Cyrille Chery, Anne-Sophie Sikora, Annick Gervais, Christine Carapito

2023, 13(5): 494-502. doi: 10.1016/j.jpha.2023.03.009

Abstract(499) HTML Full Text PDF(22) [Cited by] (7)

Abstract:
Monitoring of host cell proteins (HCPs) during the manufacturing of monoclonal antibodies (mAb) has become a critical requirement to provide effective and safe drug products. Enzyme-linked immunosorbent assays are still the gold standard methods for the quantification of protein impurities. However,...

Comprehensive and deep profiling of the plasma proteome with protein corona on zeolite NaY

Congcong Ma, Yanwei Li, Jie Li, Lei Song, Liangyu Chen, Na Zhao, Xueping Li, Ning Chen, Lixia Long, Jin Zhao, Xin Hou, Li Ren, Xubo Yuan

2023, 13(5): 503-513. doi: 10.1016/j.jpha.2023.04.002

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Abstract:
Proteomic characterization of plasma is critical for the development of novel pharmacodynamic biomarkers. However, the vast dynamic range renders the profiling of proteomes extremely challenging. Here, we synthesized zeolite NaY and developed a simple and rapid method to achieve comprehensive and de...

A robust luminescent assay for screening alkyladenine DNA glycosylase inhibitors to overcome DNA repair and temozolomide drug resistance

Ying-Qi Song, Guo-Dong Li, Dou Niu, Feng Chen, Shaozhen Jing, Vincent Kam Wai Wong, Wanhe Wang, Chung-Hang Leung

2023, 13(5): 514-522. doi: 10.1016/j.jpha.2023.04.010

Abstract(304) HTML Full Text PDF(12) [Cited by] (5)

Abstract:
Temozolomide (TMZ) is an anticancer agent used to treat glioblastoma, typically following radiation therapy and/or surgical resection. However, despite its effectiveness, at least 50% of patients do not respond to TMZ, which is associated with repair and/or tolerance of TMZ-induced DNA lesions. Stud...

Dock-able linear and homodetic di, tri, tetra and pentapeptide library from canonical amino acids: SARS-CoV-2 Mpro as a case study

Sarfraz Ahmad, Muhammad Usman Mirza, John F. Trant

2023, 13(5): 523-534. doi: 10.1016/j.jpha.2023.04.008

Abstract(539) HTML Full Text PDF(13) [Cited by] (3)

Abstract:
Peptide-based therapeutics are increasingly pushing to the forefront of biomedicine with their promise of high specificity and low toxicity. Although noncanonical residues can always be used, employing only the natural 20 residues restricts the chemical space to a finite dimension allowing for compr...

Online pharmaceutical process analysis of Chinese medicine using a miniature mass spectrometer: Extraction of active ingredients as an example

Wangmin Hu, Junling Hou, Wenjing Liu, Xuan Gu, Yulei Yang, Hongcai Shang, Mei Zhang

2023, 13(5): 535-543. doi: 10.1016/j.jpha.2023.03.005

Abstract(465) HTML Full Text PDF(26) [Cited by] (10)

Abstract:
The automation of traditional Chinese medicine (TCM) pharmaceuticals has driven the development of process analysis from offline to online. Most of common online process analytical technologies are based on spectroscopy, making the identification and quantification of specific ingredients still a ch...

2023 Vol. 13, No. 5