Journal of Pharmaceutical Analysis

Non-covalent binding analysis of sulfamethoxazole to human serum albumin:Fluorescence spectroscopy, UV-vis, FT-IR, voltammetric and molecular modeling

Praveen N. Naik, Sharanappa T. Nandibewoor, Shivamurthi A. Chimatadar

2015, (3): 143-152.

Abstract(139) PDF(2)

Abstract:
This study was designed to examine the interaction of sulfamethoxazole (SMZ) with human serum albumin(HSA). Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of human serum albumin by SMZ was static mechanism. The binding constant values...

Determination of diclofenac in pharmaceutical preparations by voltammetry and gas chromatography methods

Bilal Yilmaz, Ulvihan Ciltas

2015, (3): 153-160.

Abstract(123) PDF(3)

Abstract:
Rapid, sensitive and specific methods were developed for the determination of diclofenac in pharmaceutical preparations by linear sweep voltammetry (LSV) and gas chromatography (GC) with mass spectrometry (MS) detection. The linearity was established over the concentration range of 5–35μg/mL for LSV...

Comparison of conventional and supported liquid extraction methods for the determination of sitagliptin and simvastatin in rat plasma by LC-ESI-MS/MS

B. Ramesha, N. Manjulaa, S.R. Bijargib, V.U.M. Sarmaa, P. Sita Devia

2015, (3): 161-168.

Abstract(132) PDF(4)

Abstract:
Three extraction methods were compared for their efficiency to analyze sitagliptin and simvastatin in rat plasma by LC–MS/MS, including (1) liquid–liquid extraction (LLE), (2) solid phase extraction (SPE) and (3) supported liquid extraction (SLE). Comparison of recoveries of analytes with different ...

Quality evaluation of synthetic quorum sensing peptides used in R & D

Frederick Verbeke, Evelien Wynendaele, Sarah Braet, Matthias DHondt, Bart De Spiegeleer

2015, (3): 169-181.

Abstract(134) PDF(4)

Abstract:
Peptides are becoming an important class of molecules in the pharmaceutical field. Closely related peptide-impurities in peptides are inherent to the synthesis approach and have demonstrated to potentially mask biomedical experimental results. Quorum sensing peptides are attracting high interest in ...

Extraction, characterization and biological studies of phytochemicals from Mammea suriga

Mahesha M. Poojary, Kanivebagilu A.Vishnumurthy, Airody Vasudeva Adhikari

2015, (3): 182-189.

Abstract(162) PDF(3)

Abstract:
The present work involves extraction of phytochemicals from the root bark of a well-known Indian traditional medicinal plant, viz. Mammea suriga, with various solvents and evaluation of their in vitro antimicrobial and antioxidant activities using standard methods. The phytochemical analysis indicat...

Liquid chromatography-tandem mass spectrometry method for the estimation of adefovir in human plasma:Application to a pharmacokinetic study

Dipanjan Goswami, Sanjay Gurule, Arabinda Saha, Poonam Vats, Arshad Khuroo, Tausif Monif

2015, (3): 190-199.

Abstract(128) PDF(1)

Abstract:
An analytical method based on solid phase extraction was developed and validated for analysis of adefovir in human plasma. Adefovir-d4 was used as an internal standard and Synergi MAX RP80A (150 mm × 4.6 mm, 4μm) column provided the desired chromatographic separation of compounds followed by detecti...

Selective extraction of dimethoate from cucumber samples by use of molecularly imprinted microspheres

Jiao-Jiao Du, Rui-Xia Gao, Hu Yu, Xiao-Jing Li, Hui Mu

2015, (3): 200-206.

Abstract(102) PDF(0)

Abstract:
Molecularly imprinted polymers for dimethoate recognition were synthesized by the precipitation polymerization technique using methyl methacrylate (MMA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker. The morphology, adsorption and recognition properties wer...

Isolation and characterization of a degradation product in leflunomide and a validated selective stability-indicating HPLC-UV method for their quantification

Balraj Saini, Gulshan Bansal

2015, (3): 207-212.

Abstract(218) PDF(2)

Abstract:
Leflunomide (LLM) is subjected to forced degradation under conditions of hydrolysis, oxidation, dry heat, and photolysis as recommended by International Conference on Harmonization guideline Q1A(R2). In total, four degradation products (I–IV) were formed under different conditions. Products I, II an...

Application of analytical instruments in pharmaceutical analysis

2015, (3): 213-215.

Abstract(117) PDF(0)

Abstract:

2015 Vol. 5, No. 3