2023 Vol. 13, No. 7

simple-article
Preface for Special Issue: Single-Cell and Spatially Resolved Omics
2023, 13(7): 689-690. doi: 10.1016/j.jpha.2023.07.005
Abstract:
Perspective
Single-cell RNA-sequencing and subcellular spatial transcriptomics facilitate the translation of liver microphysiological systems for regulatory application
Dan Li, Zhou Fang, Qiang Shi, Nicholas Zhang, Binsheng Gong, Weida Tong, Ahmet F. Coskun, Joshua Xu
2023, 13(7): 691-693. doi: 10.1016/j.jpha.2023.06.013
Abstract:
Review paper
Dissecting the brain with spatially resolved multi-omics
Yijia Fangma, Mengting Liu, Jie Liao, Zhong Chen, Yanrong Zheng
2023, 13(7): 694-710. doi: 10.1016/j.jpha.2023.04.003
Abstract:
Recent studies have highlighted spatially resolved multi-omics technologies, including spatial genomics, transcriptomics, proteomics, and metabolomics, as powerful tools to decipher the spatial heterogeneity of the brain. Here, we focus on two major approaches in spatial transcriptomics (next-genera...
New discoveries in the field of metabolism by applying single-cell and spatial omics
Baocai Xie, Dengfeng Gao, Biqiang Zhou, Shi Chen, Lianrong Wang
2023, 13(7): 711-725. doi: 10.1016/j.jpha.2023.06.002
Abstract:
Single-cell multi-Omics (SCM-Omics) and spatial multi-Omics (SM-Omics) technologies provide state-of-the-art methods for exploring the composition and function of cell types in tissues/organs. Since its emergence in 2009, single-cell RNA sequencing (scRNA-seq) has yielded many groundbreaking new dis...
Original article
Single-cell analyses reveal cannabidiol rewires tumor microenvironment via inhibiting alternative activation of macrophage and synergizes with anti-PD-1 in colon cancer
Xiaofan Sun, Lisha Zhou, Yi Wang, Guoliang Deng, Xinran Cao, Bowen Ke, Xiaoqi Wu, Yanhong Gu, Haibo Cheng, Qiang Xu, Qianming Du, Hongqi Chen, Yang Sun
2023, 13(7): 726-744. doi: 10.1016/j.jpha.2023.04.013
Abstract:
Colorectal tumors often create an immunosuppressive microenvironment that prevents them from responding to immunotherapy. Cannabidiol (CBD) is a non-psychoactive natural active ingredient from the cannabis plant that has various pharmacological effects, including neuroprotective, antiemetic, anti-in...
Cux1+ proliferative basal cells promote epidermal hyperplasia in chronic dry skin disease identified by single-cell RNA transcriptomics
Minhua Huang, Ning Hua, Siyi Zhuang, Qiuyuan Fang, Jiangming Shang, Zhen Wang, Xiaohua Tao, Jianguo Niu, Xiangyao Li, Peilin Yu, Wei Yang
2023, 13(7): 745-759. doi: 10.1016/j.jpha.2023.04.004
Abstract:
Pathological dry skin is a disturbing and intractable healthcare burden, characterized by epithelial hyperplasia and severe itch. Atopic dermatitis (AD) and psoriasis models with complications of dry skin have been studied using single-cell RNA sequencing (scRNA-seq). However, scRNA-seq analysis of ...
Single-cell analysis of cellular heterogeneity and interactions in the ischemia-reperfusion injured mouse intestine
Lianhong Yin, Meng Gao, Lina Xu, Yan Qi, Lan Han, Jinyong Peng
2023, 13(7): 760-775. doi: 10.1016/j.jpha.2023.02.002
Abstract:
Nine major cell populations among 46,716 cells were identified in mouse intestinal ischemia‒reperfusion (II/R) injury by single-cell RNA sequencing. For enterocyte cells, 11 subclusters were found, in which enterocyte cluster 1 (EC1), enterocyte cluster 3 (EC3), and enterocyte cluster 8 (EC8) were n...
Discovering metabolic vulnerability using spatially resolved metabolomics for antitumor small molecule-drug conjugates development as a precise cancer therapy strategy
Xiangyi Wang, Jin Zhang, Kailu Zheng, Qianqian Du, Guocai Wang, Jianpeng Huang, Yanhe Zhou, Yan Li, Hongtao Jin, Jiuming He
2023, 13(7): 776-787. doi: 10.1016/j.jpha.2023.02.010
Abstract:
Against tumor-dependent metabolic vulnerability is an attractive strategy for tumor-targeted therapy. However, metabolic inhibitors are limited by the drug resistance of cancerous cells due to their metabolic plasticity and heterogeneity. Herein, choline metabolism was discovered by spatially resolv...
The role of signaling crosstalk of microglia in hippocampus on progression of ageing and Alzheimer's disease
He Li, Tianyuan Ye, Xingyang Liu, Rui Guo, Xiuzhao Yang, Yangyi Li, Dongmei Qi, Yihua Wei, Yifan Zhu, Lei Wen, Xiaorui Cheng
2023, 13(7): 788-805. doi: 10.1016/j.jpha.2023.05.008
Abstract:
Based on single-cell sequencing of the hippocampi of 5× familiar Alzheimer's disease (5× FAD) and wild type mice at 2-, 12-, and 24-month of age, we found an increased percentage of microglia in aging and Alzheimer's disease (AD) mice. Blood brain barrier injury may also have contributed to this inc...
A Scd1-mediated metabolic alteration participates in liver responses to low-dose bavachin
Pan Shen, Zhi-Jie Bai, Lei Zhou, Ning-Ning Wang, Zhe-Xin Ni, De-Zhi Sun, Cong-Shu Huang, Yang-Yi Hu, Cheng-Rong Xiao, Wei Zhou, Bo-Li Zhang, Yue Gao
2023, 13(7): 806-816. doi: 10.1016/j.jpha.2023.03.010
Abstract:
Hepatotoxicity induced by bioactive constituents in traditional Chinese medicines or herbs, such as bavachin (BV) in Fructus Psoraleae, has a prolonged latency to overt drug-induced liver injury in the clinic. Several studies have described BV-induced liver damage and underlying toxicity mechanisms,...
Single-cell transcriptome analysis reveals the regulatory effects of artesunate on splenic immune cells in polymicrobial sepsis
Jiayun Chen, Xueling He, Yunmeng Bai, Jing Liu, Yin Kwan Wong, Lulin Xie, Qian Zhang, Piao Luo, Peng Gao, Liwei Gu, Qiuyan Guo, Guangqing Cheng, Chen Wang, Jigang Wang
2023, 13(7): 817-829. doi: 10.1016/j.jpha.2023.02.006
Abstract:
Sepsis is characterized by a severe and life-threatening host immune response to polymicrobial infection accompanied by organ dysfunction. Studies on the therapeutic effect and mechanism of immunomodulatory drugs on the sepsis-induced hyperinflammatory or immunosuppression states of various immune c...